ArticleBiochimica et biophysica acta2003
Expression and characterization of the FXYD ion transport regulators for NMR structural studies in lipid micelles and lipid bilayers.
Article in Biochimica et biophysica acta, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Structure of the Na,K-ATPase regulatory protein FXYD2b in micelles: implications for membrane-water interfacial arginines.Biochimica et biophysica acta · 2015Article
- Oxidative inhibition of the vascular Na+-K+ pump via NADPH oxidase-dependent β1-subunit glutathionylation: implications for angiotensin II-induced vascular dysfunction.Free radical biology & medicine · 2013Article
- Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.Progress in nuclear magnetic resonance spectroscopy · 2009Article
- Using LongSAGE to Detect Biomarkers of Cervical Cancer Potentially Amenable to Optical Contrast Agent Labelling.Biomarker insights · 2007Article
- Redesigning channel-forming peptides: amino acid substitutions that enhance rates of supramolecular self-assembly and raise ion transport activity.Biophysical journal · 2006Article
- Correlation of gene and protein structures in the FXYD family proteins.Journal of molecular biology · 2005Article
- Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins.Protein science : a publication of the Protein Society · 2005Article
- Structural studies of apoptosis and ion transport regulatory proteins in membranes.Magnetic resonance in chemistry : MRC · 2004Article
- Hydration-optimized oriented phospholipid bilayer samples for solid-state NMR structural studies of membrane proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2003Article
- Serine 68 phospholemman phosphorylation during forskolin-induced swine carotid artery relaxation.Journal of vascular researchArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The proteins PLM (phospholemman), CHIF (channel inducing factor), and Mat8 (mammary tumor protein 8 kDa) are members of the FXYD family of ion transport regulatory membrane proteins. Here we describe their cloning and expression in Escherichia coli, and their purification for NMR structural studies in lipid micelles and lipid bilayers. The molecular masses of the purified recombinant FXYD proteins, determined from SDS-PAGE and from MALDI TOF mass spectrometry, reflect monomeric species. The solution NMR and CD spectra in SDS micelles show that they adopt helical conformations. The solid-state NMR spectra in lipid bilayers give the first view of their transmembrane architecture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.