ArticleJournal of bacteriology2003
Characterization of the CipA scaffolding protein and in vivo production of a minicellulosome in Clostridium acetobutylicum.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 58 citations in OpenAlex.
- Cellulolytic and Xylanolytic Enzymes from Yeasts: Properties and Industrial Applications.Molecules (Basel, Switzerland) · 2022Review
- Quantitative proteomic analysis of the influence of lignin on biofuel production by Clostridium acetobutylicum ATCC 824.Biotechnology for biofuels · 2016Article
- Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.Biotechnology for biofuels · 2016Article
- Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.Biotechnology for biofuels · 2013Article
- Effects of synthetic cohesin-containing scaffold protein architecture on binding dockerin-enzyme fusions on the surface of Lactococcus lactis.Microbial cell factories · 2012Article
- Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome.Applied and environmental microbiology · 2012Article
- Natural strategies for the spatial optimization of metabolism in synthetic biology.Nature chemical biology · 2012Review
- Prokaryotic gene clusters: a rich toolbox for synthetic biology.Biotechnology journal · 2010Review
- Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis.Microbial cell factories · 2010Article
- Cellulase, clostridia, and ethanol.Microbiology and molecular biology reviews : MMBR · 2005Review
- Heterologous production, assembly, and secretion of a minicellulosome by Clostridium acetobutylicum ATCC 824.Applied and environmental microbiology · 2005Article
- Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800.Applied and environmental microbiology · 2004Article
- Production of heterologous and chimeric scaffoldins by Clostridium acetobutylicum ATCC 824.Journal of bacteriology · 2004Article
- Cellulosomes from mesophilic bacteria.Journal of bacteriology · 2003Review
- Microbial conversion of corn stalks to riches.Journal of bacteriology · 2003Article
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2 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The cipA gene encoding the Clostridium acetobutylicum scaffolding protein CipA was cloned and expressed in Escherichia coli. CipA contains an N-terminal signal peptide, a family 3a cellulose-binding domain (CBD), five type I cohesin domains, and six hydrophilic domains. The uniqueness of CipA lies in the enchainment of cohesin domains that are all separated by a hydrophilic domain. Affinity-purified CipA was used in equilibrium-binding experiments to characterize the interaction of CipA with crystalline cellulose. A K(d) of 0.038 micro M and a [C](max) of 0.43 micro mol of CipA bound per g of Avicel were determined. A mini-CipA polypeptide consisting of a CBD3a and two cohesin domains was overexpressed in C. acetobutylicum, yielding the in vivo formation of a minicellulosome. This is to our knowledge the first demonstration of the in vivo assembly of a recombinant minicellulosome.
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