ArticleJournal of bacteriology2003
Heme-responsive transcriptional activation of Bordetella bhu genes.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 60 citations in OpenAlex.
- The Sigma Factor AsbI Is Required for the Expression of Acinetobactin Siderophore Transport Genes inInternational journal of molecular sciences · 2023Article
- Carbon Monoxide-Sensing Transcription Factors: Regulators of Microbial Carbon Monoxide Oxidation Pathway Gene Expression.Journal of bacteriology · 2023Review
- Severe anaemia, iron deficiency, and susceptibility to invasive bacterial infections.Wellcome open research · 2023Review
- Axial Heme Coordination by the Tyr-His Motif in the Extracellular Hemophore HasAp Is Critical for the Release of Heme to the HasR Receptor ofBiochemistry · 2021Article
- Contributions of the heme coordinating ligands of theThe Journal of biological chemistry · 2020Article
- Post-transcriptional regulation of theThe Journal of biological chemistry · 2019Article
- Transcriptional and posttranscriptional regulation of Shigella shuT in response to host-associated iron availability and temperature.MicrobiologyOpen · 2017Article
- Interspecies variations in Bordetella catecholamine receptor gene regulation and function.Infection and immunity · 2015Article
- Iron homeostasis in theAdvances in botanical research · 2013Article
- Role and regulation of heme iron acquisition in gram-negative pathogens.Frontiers in cellular and infection microbiology · 2013Review
- HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum.Journal of bacteriology · 2012Article
- Involvement of multiple distinct Bordetella receptor proteins in the utilization of iron liberated from transferrin by host catecholamine stress hormones.Molecular microbiology · 2012Article
- The Neisseria meningitidis ZnuD zinc receptor contributes to interactions with epithelial cells and supports heme utilization when expressed in Escherichia coli.Infection and immunity · 2012Article
- Factors influencing the diversity of iron uptake systems in aquatic microorganisms.Frontiers in microbiology · 2012Article
- Transcriptional profiling of the iron starvation response in Bordetella pertussis provides new insights into siderophore utilization and virulence gene expression.Journal of bacteriology · 2011Article
- The battle for iron between bacterial pathogens and their vertebrate hosts.PLoS pathogens · 2010Review
- Characterization of a heme-regulated non-coding RNA encoded by the prrF locus of Pseudomonas aeruginosa.PloS one · 2010Article
- Expression of BfrH, a putative siderophore receptor of Bordetella bronchiseptica, is regulated by iron, Fur1, and the extracellular function sigma factor EcfI.Infection and immunity · 2010Article
- Regulatory role of the MisR/S two-component system in hemoglobin utilization in Neisseria meningitidis.Infection and immunity · 2010Article
- Microbial telesensing: probing the environment for friends, foes, and food.Cell host & microbe · 2009Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bordetella pertussis and Bordetella bronchiseptica, gram-negative respiratory pathogens of mammals, possess a heme iron utilization system encoded by the bhuRSTUV genes. Preliminary evidence suggested that expression of the BhuR heme receptor was stimulated by the presence of heme under iron-limiting conditions. The hurIR (heme uptake regulator) genes were previously identified upstream of the bhuRSTUV gene cluster and are predicted to encode homologs of members of the iron starvation subfamily of extracytoplasmic function (ECF) regulators. In this study, B. pertussis and B. bronchiseptica DeltahurI mutants, predicted to lack an ECF sigma factor, were constructed and found to be deficient in the utilization of hemin and hemoglobin. Genetic complementation of DeltahurI strains with plasmid-borne hurI restored wild-type levels of heme utilization. B. bronchiseptica DeltahurI mutant BRM23 was defective in heme-responsive production of the BhuR heme receptor; hurI in trans restored heme-inducible BhuR expression to the mutant and resulted in BhuR overproduction. Transcriptional analyses with bhuR-lacZ fusion plasmids confirmed that bhuR transcription was activated in iron-starved cells in response to heme compounds. Heme-responsive bhuR transcription was not observed in mutant BRM23, indicating that hurI is required for positive regulation of bhu gene expression. Furthermore, bhuR was required for heme-inducible bhu gene activation, supporting the hypothesis that positive regulation of bhuRSTUV occurs by a surface signaling mechanism involving the heme-iron receptor BhuR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.