ArticleJournal of bacteriology2003
Characterization, expression, and mutation of the Lactococcus lactis galPMKTE genes, involved in galactose utilization via the Leloir pathway.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 74 citations in OpenAlex.
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- The Discovery of GALM Deficiency (Type IV Galactosemia) and Newborn Screening System for Galactosemia in Japan.International journal of neonatal screening · 2021Review
- Quantitative Proteomic Analysis of the Response of Probiotic PutativeFrontiers in microbiology · 2019Article
- Altering textural properties of fermented milk by using surface-engineered Lactococcus lactis.Microbial biotechnology · 2018Article
- Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei.Microbial cell factories · 2018Article
- Further Elucidation of Galactose Utilization inFrontiers in microbiology · 2018Article
- Transcription factor Rex in regulation of pathophysiology in oral pathogens.Molecular oral microbiology · 2016Review
- Lantibiotic resistance.Microbiology and molecular biology reviews : MMBR · 2015Review
- The carbohydrate metabolism signature of lactococcus lactis strain A12 reveals its sourdough ecosystem origin.Applied and environmental microbiology · 2013Article
- A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363.Applied and environmental microbiology · 2012Article
- Towards enhanced galactose utilization by Lactococcus lactis.Applied and environmental microbiology · 2010Article
- Utilization of lactose and galactose by Streptococcus mutans: transport, toxicity, and carbon catabolite repression.Journal of bacteriology · 2010Article
- Production of functionalized biopolyester granules by recombinant Lactococcus lactis.Applied and environmental microbiology · 2009Article
- Reduced lysis upon growth of Lactococcus lactis on galactose is a consequence of decreased binding of the autolysin AcmA.Applied and environmental microbiology · 2008Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cluster containing five similarly oriented genes involved in the metabolism of galactose via the Leloir pathway in Lactococcus lactis subsp. cremoris MG1363 was cloned and characterized. The order of the genes is galPMKTE, and these genes encode a galactose permease (GalP), an aldose 1-epimerase (GalM), a galactokinase (GalK), a hexose-1-phosphate uridylyltransferase (GalT), and a UDP-glucose 4-epimerase (GalE), respectively. This genetic organization reflects the order of the metabolic conversions during galactose utilization via the Leloir pathway. The functionality of the galP, galK, galT, and galE genes was shown by complementation studies performed with both Escherichia coli and L. lactis mutants. The GalP permease is a new member of the galactoside-pentose-hexuronide family of transporters. The capacity of GalP to transport galactose was demonstrated by using galP disruption mutant strains of L. lactis MG1363. A galK deletion was constructed by replacement recombination, and the mutant strain was not able to ferment galactose. Disruption of the galE gene resulted in a deficiency in cell separation along with the appearance of a long-chain phenotype when cells were grown on glucose as the sole carbon source. Recovery of the wild-type phenotype for the galE mutant was obtained either by genetic complementation or by addition of galactose to the growth medium.
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