ArticleThe Biochemical journal2003
Functional characterization of the non-catalytic ectodomains of the nucleotide pyrophosphatase/phosphodiesterase NPP1.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- ENPP1 deficiency: A clinical update on the relevance of individual variants using a locus-specific patient database.Human mutation · 2022Review
- Mutation update: Variants of the ENPP1 gene in pathologic calcification, hypophosphatemic rickets, and cutaneous hypopigmentation with punctate keratoderma.Human mutation · 2022Review
- Enzymology of extracellular NAD metabolism.Cellular and molecular life sciences : CMLS · 2021Review
- Crystal structures of human ENPP1 in apo and bound forms.Acta crystallographica. Section D, Structural biology · 2020Article
- Crystal structure and substrate binding mode of ectonucleotide phosphodiesterase/pyrophosphatase-3 (NPP3).Scientific reports · 2018Article
- The hepatic ectonucleotide pyrophosphatase/phosphodiesterase 1 gene mRNA abundance is reduced by insulin and induced by dexamethasone.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2018Article
- Autotaxin, a lysophospholipase D with pleomorphic effects in oncogenesis and cancer progression.Journal of lipid research · 2016Review
- EndoU is a novel regulator of AICD during peripheral B cell selection.The Journal of experimental medicine · 2014Article
- Effects of pH on the production of phosphate and pyrophosphate by matrix vesicles' biomimetics.Calcified tissue international · 2013Article
- Variants of insulin-signaling inhibitor genes in type 2 diabetes and related metabolic abnormalities.International journal of genomics · 2013Article
- Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cellular function and molecular structure of ecto-nucleotidases.Purinergic signalling · 2012Review
- Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.The Journal of biological chemistry · 2010Article
- Autotaxin.Cellular and molecular life sciences : CMLS · 2009Review
- Domain interplay mediated by an essential disulfide linkage is critical for the activity and secretion of the metastasis-promoting enzyme autotaxin.The Journal of biological chemistry · 2009Article
- Activity and expression of ecto-nucleotide pyrophosphate/phosphodiesterases in a hepatic stellate cell line.Molecular and cellular biochemistry · 2009Article
- Lysophospholipid interactions with protein targets.Biochimica et biophysica acta · 2008Review
- The role of membrane glycoprotein plasma cell antigen 1/ectonucleotide pyrophosphatase phosphodiesterase 1 in the pathogenesis of insulin resistance and related abnormalities.Endocrine reviews · 2008Review
- Biochemical characterization of ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP, E.C. 3.1.4.1) from rat heart left ventricle.Molecular and cellular biochemistry · 2007Article
- Modulation of purinergic signaling by NPP-type ectophosphodiesterases.Purinergic signalling · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitous nucleotide pyrophosphatases/phosphodiesterases NPP1-3 consist of a short intracellular N-terminal domain, a single transmembrane domain and a large extracellular part, comprising two somatomedin-B-like domains, a catalytic domain and a poorly defined C-terminal domain. We show here that the C-terminal domain of NPP1-3 is structurally related to a family of DNA/RNA non-specific endonucleases. However, none of the residues that are essential for catalysis by the endonucleases are conserved in NPP1-NPP3, suggesting that the nuclease-like domain of NPP1-3 does not represent a second catalytic domain. Truncation analysis revealed that the nuclease-like domain of NPP1 is required for protein stability, for the targeting of NPP1 to the plasma membrane and for the expression of catalytic activity. We also demonstrate that 16 conserved cysteines in the somatomedin-B-like domains of NPP1, in concert with two flanking cysteines, mediate the dimerization of NPP1. The K173Q polymorphism of NPP1, which maps to the second somatomedin-B-like domain and has been associated with the aetiology of insulin resistance, did not affect the dimerization or catalytic activity of NPP1, and did not endow NPP1 with an affinity for the insulin receptor. Our data suggest that the non-catalytic ectodomains contribute to the subunit structure, stability and function of NPP1-3.
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