ArticleWorld journal of gastroenterology2003
HCV replication in PBMC and its influence on interferon therapy.
Article in World journal of gastroenterology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Chronic hepatitis C treatment outcomes in low- and middle-income countries: a systematic review and meta-analysis.Bulletin of the World Health Organization · 2012Pooled it
- The IFN-λ4 Conundrum: When a Good Interferon Goes Bad.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2019Review
- MicroRNA Profile of HCV Spontaneous Clarified Individuals, Denotes Previous HCV Infection.Journal of clinical medicine · 2019Article
- Analysis of mutations in the core and NS5A genes of hepatitis C virus in non-responder and relapser patients after treatment with Peg-IFN-α and ribavirin.Virusdisease · 2016Article
- Evidence of distinct populations of hepatitis C virus in the liver and plasma of patients co-infected with HIV and HCV.Journal of medical virology · 2014Article
- Molecular epidemiology of different hepatitis C genotypes in serum and peripheral blood mononuclear cells in jahrom city of iran.Hepatitis monthly · 2014Article
- In vitro inhibition of hepatitis C virus by antisense oligonucleotides in PBMC compared to hepatoma cells.BioMed research international · 2014Article
- Hepatitis C virus infection of a thyroid cell line: implications for pathogenesis of hepatitis C virus and thyroiditis.Thyroid : official journal of the American Thyroid Association · 2013Article
- Human Interferon Regulatory Factor 2 Gene Expression is Induced in Chronic Hepatitis C Virus Infection-A Possible Mode of Viral Persistence.Journal of clinical and experimental hepatology · 2012Article
- Analysis of a non-structural gene reveals evidence of possible hepatitis C virus (HCV) compartmentalization.Journal of medical virology · 2012Article
- Human cell types important for hepatitis C virus replication in vivo and in vitro: old assertions and current evidence.Virology journal · 2011Review
- HCV infection and B-cell lymphomagenesis.Advances in hematology · 2011Article
- Distribution of different hepatitis C virus genotypes in patients with hepatitis C virus infection.World journal of gastroenterology · 2010Article
- Hepatitis C virus lymphotropism and peculiar immunological phenotype: effects on natural history and antiviral therapy.World journal of gastroenterology · 2009Article
- Whole blood as an alternative to plasma for detection of hepatitis C virus RNA.Journal of clinical microbiology · 2008Article
- Compartmentalization of hepatitis C virus (HCV) during HCV/HIV coinfection.The Journal of infectious diseases · 2007Article
- Soluble egg antigen of Schistosoma Haematobium induces HCV replication in PBMC from patients with chronic HCV infection.BMC infectious diseases · 2006Article
- Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: binding and entry.World journal of gastroenterology · 2005Article
- Management of Hepatitis C in HIV-infected Patients.Current infectious disease reports · 2005Article
- Monocyte chemotactic protein-1 and soluble adhesion molecules as possible prognostic markers of the efficacy of antiviral treatment in chronic hepatitis C.World journal of gastroenterology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study hepatic virus C (HCV) RNA and HCV protein expression in peripheral blood mononuclear cells (PBMCs) of patients with HCV infection, and explore the relationship between the HCV RNA in the PBMCs and response to interferon (IFN) therapy.
methodsType-specific primers were designed and RT-nested PCR was used to detect the plus- and minus- strands of HCV RNA in PBMCs of 54 patients with HCV infection; Indirect immunofluorescence assay was applied to identify HCVNS5 protein expression in PBMCs; 6 month-, 3 MU-IFN regiment was administrated to observe the responses to IFN in 35 chronic hepatitis C patients with different HCV RNA status in PBMCs.
resultsHCV plus strand RNA was found in 10 of 19 (52.6 %) acute hepatitis C patients and 22 of 35 (62.9 %) chronic hepatitis C patients. HCV minus strand RNA was detected in 14 of 35 (40.0 %) chronic hepatitis C patients, but only one patient (5.3 %) with acute HCV infection was found to be minus HCV RNA positive. Though no HCV NS5 protein expression was found in the examined 10 cases of acute HCV infection, it was positive in 17 of 20 (85.0 %) chronic hepatitis C patients by indirect immunofluorescence assay. There are significant differences of positive rate of the minus-strand and HCVNS5 protein between acute and chronic hepatitis C groups (u=2.07, P<0.05 and u=4.43, P<0.01 respectively). The patients with minus-strand HCV RNA showed a significantly lower 6-month sustained response (SR-6) to IFN compared to those without minus-strand HCVRNA in PBMCs (biologically 14.3 % vs 42.8 %, chi(2)=4.12, P<0.05 and virologically 7.1 % vs 23.9 %, chi(2)=4.24, P<0.05).
conclusionHCV is capable of infecting and replicating in PBMCs, and HCVNS5 protein was expressed in PBMCs. The patients with minus strand HCV RNA in PBMCs showed a significantly lower 6-month sustained response to IFN, suggesting that minus-strand HCV RNA in PBMCs may be one of the factors influencing response to IFN therapy.
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Registered trials
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