ArticlePlant physiology2003
Do phytotropins inhibit auxin efflux by impairing vesicle traffic?
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 123 citations in OpenAlex.
- The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii.The New phytologist · 2025Article
- Naphthylphthalamic acid associates with and inhibits PIN auxin transporters.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Local auxin biosynthesis is required for root regeneration after wounding.Nature plants · 2020Article
- Butylated Hydroxytoluene (BHT) Inhibits PIN1 Exocytosis From BFA Compartments in Arabidopsis Roots.Frontiers in plant science · 2020Article
- Article
- Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.BMC plant biology · 2019Article
- Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway.Scientific reports · 2019Article
- ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties.Journal of experimental botany · 2017Article
- Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte AlgaPlant physiology · 2017Article
- cis-Cinnamic Acid Is a Novel, Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation.Plant physiology · 2017Article
- Auxin flow-mediated competition between axillary buds to restore apical dominance.Scientific reports · 2016Article
- Integrated role of ROS and CaProtoplasma · 2016Article
- Inhibitors of plant hormone transport.Protoplasma · 2016Review
- The Allelochemical MDCA Inhibits Lignification and Affects Auxin Homeostasis.Plant physiology · 2016Article
- Ethylene- and Shade-Induced Hypocotyl Elongation Share Transcriptome Patterns and Functional Regulators.Plant physiology · 2016Article
- Dynamics and control of phloem loading of indole-3-acetic acid in seedling cotyledons of Ricinus communis.Journal of experimental botany · 2016Article
- TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.The Plant cell · 2016Article
- NtGNL1a ARF-GEF acts in endocytosis in tobacco cells.BMC plant biology · 2015Article
- Current insights into hormonal regulation of microspore embryogenesis.Frontiers in plant science · 2015Review
- Polar auxin transport is essential for medial versus lateral tissue specification and vascular-mediated valve outgrowth in Arabidopsis gynoecia.Plant physiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytotropins such as 1-N-naphthylphthalamic acid (NPA) strongly inhibit auxin efflux, but the mechanism of this inhibition remains unknown. Auxin efflux is also strongly decreased by the vesicle trafficking inhibitor brefeldin A (BFA). Using suspension-cultured interphase cells of the BY-2 tobacco (Nicotiana tabacum L. cv Bright-Yellow 2) cell line, we compared the effects of NPA and BFA on auxin accumulation and on the arrangement of the cytoskeleton and endoplasmic reticulum (ER). The inhibition of auxin efflux (stimulation of net accumulation) by both NPA and BFA occurred rapidly with no measurable lag. NPA had no observable effect on the arrangement of microtubules, actin filaments, or ER. Thus, its inhibitory effect on auxin efflux was not mediated by perturbation of the cytoskeletal system and ER. BFA, however, caused substantial alterations to the arrangement of actin filaments and ER, including a characteristic accumulation of actin in the perinuclear cytoplasm. Even at saturating concentrations, NPA inhibited net auxin efflux far more effectively than did BFA. Therefore, a proportion of the NPA-sensitive auxin efflux carriers may be protected from the action of BFA. Maximum inhibition of auxin efflux occurred at concentrations of NPA substantially below those previously reported to be necessary to perturb vesicle trafficking. We found no evidence to support recent suggestions that the action of auxin transport inhibitors is mediated by a general inhibition of vesicle-mediated protein traffic to the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.