ArticlePlant physiology2003
Ethylmethanesulfonate saturation mutagenesis in Arabidopsis to determine frequency of herbicide resistance.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.
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Who cites it
58 citing papers in PubMed, 1 synthesis or guideline pooled it, 174 citations in OpenAlex.
- Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants.Frontiers in plant science · 2020Pooled it
- Thirty years of glyphosate-resistant crops and weeds: Current situation and future prospects.Pest management science · 2026Review
- Alternative oxidase and ethylene form a positive feed-forward loop in mitochondrial retrograde signaling.Plant communications · 2026Article
- Telomere-to-telomere genome assembly and comprehensive mutation library facilitate bitter gourd breeding and functional genomics research.Plant communications · 2026Article
- Genomic and Functional Characterization of Acetolactate Synthase (ALS) Genes in Stress Adaptation of the Noxious WeedPlants (Basel, Switzerland) · 2025Article
- AtCHE1, the Arabidopsis homolog of mammalian AATF/Che-1 protein, is involved in safeguarding genome stability.Communications biology · 2025Article
- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- Induced genetic diversity through mutagenesis in wheat gene pool and significant use of SCoT markers to underpin key agronomic traits.BMC plant biology · 2024Article
- Changes in Metabolite Profiling and Expression Levels of Key Genes Involved in the Terpenoid Biosynthesis Pathway in Garden Sage (Metabolites · 2023Article
- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Point mutations that boost aromatic amino acid production and COScience advances · 2022Article
- Construction of a density mutant collection in bitter gourdFrontiers in plant science · 2022Article
- Molecular and Biochemical Evaluation of Ethyl Methanesulfonate-Induced Mutant Lines inIranian journal of biotechnology · 2022Article
- CRISPR screens in plants: approaches, guidelines, and future prospects.The Plant cell · 2021Review
- Review
- Evolution of Glyphosate-Resistant Weeds.Reviews of environmental contamination and toxicology · 2021Review
- Less Is More: a Mutation in the Chemical Defense Pathway of Erysimum cheiranthoides (Brassicaceae) Reduces Total Cardenolide Abundance but Increases Resistance to Insect Herbivores.Journal of chemical ecology · 2020Article
- A Genetic Screen to Identify New Molecular Players Involved in Photoprotection qH inPlants (Basel, Switzerland) · 2020Article
- Mechanisms of evolved herbicide resistance.The Journal of biological chemistry · 2020Review
- Next-generation forward genetic screens: using simulated data to improve the design of mapping-by-sequencing experiments in Arabidopsis.Nucleic acids research · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant resistance to glyphosate has been reported far less frequently than resistance to sulfonylurea and imidazolinone herbicides. However, these studies tend to be anecdotal, without side by side comparisons for a single species or natural isolate. In this study, we tested the frequencies of resistance of three herbicides in a controlled ethylmethanesulfonate (EMS) saturation mutagenesis experiment, allowing a direct comparison of the frequencies at which resistant mutant plants arise. The 100% growth inhibition dose rates of glyphosate, chlorsulfuron (a sulfonylurea herbicide), and imazethapyr (an imidazolinone herbicide) were determined for Arabidopsis. Populations of EMS-mutagenized M(2) seedlings were sprayed with twice the 100% growth inhibition dose of glyphosate, chlorsulfuron, or imazethapyr, and herbicide-resistant mutants were identified. Although there were no glyphosate-resistant mutants among M(2) progeny of 125,000 Columbia and 125,000 Landsberg erecta M(1) lines, chlorsulfuron resistance and imazethapyr resistance each appeared at frequencies of 3.2 x 10(-5). Given the observed frequency of herbicide resistance mutations, we calculate that there are at least 700 mutations in each EMS-mutagenized Arabidopsis line and that fewer than 50,000 M(1) lines are needed to have a 95% chance of finding a mutation in any given G:C base pair in the genome. As part of this study, two previously unreported Arabidopsis mutations conferring resistance to imidazolinone herbicides, csr1-5 (Ala-122-Thr) and csr1-6 (Ala-205-Val), were discovered. Neither of these mutations caused enhanced resistance to chlorsulfuron in Arabidopsis.
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