ArticlePlant physiology2003
The effects of polyethylene glycol on gene expression of developing white spruce somatic embryos.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 143 citations in OpenAlex.
- Ectomycorrhizal Symbiosis as a Bio-Enhancement Strategy for Transplantation of Somatic Embryo-DerivedPlants (Basel, Switzerland) · 2026Article
- Stage-specific metabolic divergence in flavonoid biosynthesis correlates with embryogenic capacity in rubber tree (Frontiers in plant science · 2026Article
- Regulatory mechanisms of the LBD40 transcription factor inPlant direct · 2023Article
- Transduction of Signals during Somatic Embryogenesis.Plants (Basel, Switzerland) · 2022Review
- Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status.Frontiers in plant science · 2022Article
- In Vitro Stress-Mediated Somatic Embryogenesis in Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Influence of Polyethylene Glycol on Leaf Anatomy, Stomatal Behavior, Water Loss, and Some Physiological Traits of Date Palm Plantlets GrownPlants (Basel, Switzerland) · 2020Article
- Morphological analyses and variation in carbohydrate content during the maturation of somatic embryos ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Somatic embryogenesis in guava (Psidium guajava L.): current status and future perspectives.3 Biotech · 2017Review
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- Proteomic analysis of stress-related proteins and metabolic pathways in Picea asperata somatic embryos during partial desiccation.Plant biotechnology journal · 2017Article
- Desiccation Treatment and Endogenous IAA Levels Are Key Factors Influencing High Frequency Somatic Embryogenesis inFrontiers in plant science · 2017Article
- Norway spruce embryogenesis: changes in carbohydrate profile, structural development and response to polyethylene glycol.Tree physiology · 2016Article
- Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine).Genetics and molecular biology · 2012Article
- Large impact of the apoplast on somatic embryogenesis in Cyclamen persicum offers possibilities for improved developmental control in vitro.BMC plant biology · 2010Article
- LEAFY COTYLEDON2 gene expression and auxin treatment in relation to embryogenic capacity of Arabidopsis somatic cells.Plant cell reports · 2009Article
- Transcriptome analysis during somatic embryogenesis of the tropical monocot Elaeis guineensis: evidence for conserved gene functions in early development.Plant molecular biology · 2009Article
- Genome-wide transcriptional analysis of super-embryogenic Medicago truncatula explant cultures.BMC plant biology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic embryogenic cultures of white spruce (Picea glauca) represent a valuable system to study molecular mechanisms regulating embryo development because many embryos of defined developmental stages can be generated. The inclusion of polyethylene glycol (PEG) in the maturation medium can improve the number and quality of embryos produced. To learn more about the mechanism of action of PEG, we analyzed transcript profiles of stage-specific embryos matured without (control) or with (PEG treated) PEG. RNA extracted from maturing spruce embryos was analyzed on DNA microarrays containing 2,178 cDNAs from loblolly pine (Pinus taeda). The efficiency of heterologous hybridization between spruce and pine species on microarrays has been documented previously (L. van Zyl, S. von Arnold, P. Bozhkov, Y. Chen, U. Egertsdotter, J. MacKay, R. Sederoff, J. Shen, L. Zelena, D. Clapham [2002] Comp Funct Genomics 3: 306-318). Several pine genes, including the apparent homologs to the Arabidopsis genes ZWILLE, FIDDLEHEAD, FUSCA, and SCARECROW, increased in expression after PEG treatments. These genes are known to be involved in the formation of the embryo body plan and in the control of the shoot and root apical meristems. The increased transcript levels of these genes in immature PEG-treated embryos suggest that PEG may improve the quality of spruce somatic embryos by promoting normal differentiation of the embryonic shoot and root. Changes in the transcript levels of many genes involved in sucrose catabolism and nitrogen assimilation and utilization were also observed between control and PEG-treated embryos.
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