ArticleMolecular biology of the cell2003
The identification of pats1, a novel gene locus required for cytokinesis in Dictyostelium discoideum.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
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- Roco Proteins and the Parkinson's Disease-Associated LRRK2.International journal of molecular sciences · 2018Review
- LRRK 2 gene mutations in the pathophysiology of the ROCO domain and therapeutic targets for Parkinson's disease: a review.Journal of biomedical science · 2018Review
- Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division.Scientific reports · 2017Article
- Genetic, structural, and molecular insights into the function of ras of complex proteins domains.Chemistry & biology · 2014Review
- The LRRK2-related Roco kinase Roco2 is regulated by Rab1A and controls the actin cytoskeleton.Molecular biology of the cell · 2011Article
- A QUICK screen for Lrrk2 interaction partners--leucine-rich repeat kinase 2 is involved in actin cytoskeleton dynamics.Molecular & cellular proteomics : MCP · 2011Article
- The ROCO kinase QkgA is necessary for proliferation inhibition by autocrine signals in Dictyostelium discoideum.Eukaryotic cell · 2010Article
- A phylogenetic survey of myotubularin genes of eukaryotes: distribution, protein structure, evolution, and gene expression.BMC evolutionary biology · 2010Article
- Characterization of the Roco protein family in Dictyostelium discoideum.Eukaryotic cell · 2010Article
- LRRK2 in Parkinson's disease: in vivo models and approaches for understanding pathogenic roles.The FEBS journal · 2009Review
- The Roc domain of leucine-rich repeat kinase 2 is sufficient for interaction with microtubules.Journal of neuroscience research · 2008Article
- The dictyostelium kinome--analysis of the protein kinases from a simple model organism.PLoS genetics · 2006Article
- Rapid generation of gene disruption constructs by in vitro transposition and identification of a Dictyostelium protein kinase that regulates its rate of growth and development.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we describe the identification and characterization of the cytokinesis-deficient mutant cell line 17HG5, which was generated in a restriction enzyme-mediated integration mutagenesis screen designed to isolate genes required for cytokinesis in Dictyostelium discoideum. Phenotypic characterization of the 17HG5 cell line revealed no apparent defects in the global functionality of the actomyosin cytoskeleton except for the observed cytokinesis defect when grown in suspension culture. Plasmid rescue was used to identify the disrupted gene locus (pats1; protein associated with the transduction of signal 1). that caused the cytokinesis defect. Disruption of the pats1 locus was recreated through homologous recombination in several independent cell lines, each recapitulating the cytokinesis-defective phenotype and thereby confirming that this gene locus is important for proper cytokinesis. Sequence data obtained by analysis of the genomic region flanking the inserted restriction enzyme-mediated integration plasmid revealed an 8892-bp genomic open reading frame encoding a 2964-amino-acid protein. The putative pats1 protein contains 3 regulatory domains (RI-phosphatase, RII-GTP-binding, R-III protein kinase), 13 leucine-rich repeats, and 8 WD-40 repeats. These regulatory domains coupled with the protein-protein interacting domains suggest that pats1 is involved in signal transduction during cytokinesis in Dictyostelium.
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