ArticleMolecular biology of the cell2003
N-terminal domain of yeast telomerase reverse transcriptase: recruitment of Est3p to the telomerase complex.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 79 citations in OpenAlex.
- Cryo-electron microscopy structure of the budding yeast telomerase holoenzyme.Science (New York, N.Y.) · 2026Article
- The Missing Piece: Functional Telomerase Restored in the Beetle Model.Genome biology and evolution · 2026Article
- The canonical RPA complex interacts with Est3 to regulate yeast telomerase activity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Functional interaction between compound heterozygous TERT mutations causes severe telomere biology disorder.Blood advances · 2022Article
- Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase.Scientific reports · 2020Article
- Proteomics of yeast telomerase identified Cdc48-Npl4-Ufd1 and Ufd4 as regulators of Est1 and telomere length.Nature communications · 2015Article
- Regulated assembly and disassembly of the yeast telomerase quaternary complex.Genes & development · 2014Article
- Identification of human TERT elements necessary for telomerase recruitment to telomeres.eLife · 2014Article
- Novel telomere-anchored PCR approach for studying sexual stage telomeres in Aspergillus nidulans.PloS one · 2014Article
- The anaphase promoting complex contributes to the degradation of the S. cerevisiae telomerase recruitment subunit Est1p.PloS one · 2013Article
- Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to end.Genetics · 2012Review
- Telomerase regulatory subunit Est3 in two Candida species physically interacts with the TEN domain of TERT and telomeric DNA.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A mutation in the catalytic subunit of yeast telomerase alters primer-template alignment while promoting processivity and protein-DNA binding.Journal of cell science · 2011Article
- Stimulation of yeast telomerase activity by the ever shorter telomere 3 (Est3) subunit is dependent on direct interaction with the catalytic protein Est2.The Journal of biological chemistry · 2011Article
- The Saccharomyces cerevisiae telomerase subunit Est3 binds telomeres in a cell cycle- and Est1-dependent manner and interacts directly with Est1 in vitro.PLoS genetics · 2011Article
- Direct involvement of the TEN domain at the active site of human telomerase.Nucleic acids research · 2011Article
- Ribonucleoprotein multimers and their functions.Critical reviews in biochemistry and molecular biology · 2010Review
- Human telomerase reverse transcriptase (hTERT) Q169 is essential for telomerase function in vitro and in vivo.PloS one · 2009Article
- A proposed OB-fold with a protein-interaction surface in Candida albicans telomerase protein Est3.Nature structural & molecular biology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomerase is a reverse transcriptase that maintains chromosome ends. The N-terminal half of the catalytic protein subunit (TERT) contains three functional domains (I, II, and III) that are conserved among TERTs but not found in other reverse transcriptases. Guided by an amino acid sequence alignment of nine TERT proteins, mutations were introduced into yeast TERT (Est2p). In support of the proposed alignment, mutation of virtually all conserved residues resulted in loss-of-function or temperature sensitivity, accompanied by telomere shortening. Overexpression of telomerase component Est3p led to allele-specific suppression of the temperature-sensitive mutations in region I, suggesting that Est3p interacts with this protein domain. As predicted by the genetic results, a lethal mutation in region I resulted in loss of Est3p from the telomerase complex. We conclude that Est2p region I is required for the recruitment of Est3p to yeast telomerase. Given the phylogenetic conservation of region I of TERT, this protein domain may provide the equivalent function in all telomerases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.