ArticleMolecular human reproduction2003
Interleukin-1beta induces glycosaminoglycan synthesis via the prostaglandin E2 pathway in cultured human cervical fibroblasts.
Article in Molecular human reproduction, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Melatonin in Pregnancy-Related Disorders: A Review of Protective Mechanisms and Therapeutic Potential.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Synovium friction properties are influenced by proteoglycan content.Journal of biomechanics · 2024Article
- Cyclooxygenase-2 Modulates Glycosaminoglycan Production in the Skin During Salt Overload.Frontiers in physiology · 2020Article
- Maternal Whole Blood Gene Expression at 18 and 28 Weeks of Gestation Associated with Spontaneous Preterm Birth in Asymptomatic Women.PloS one · 2016Article
- The interplay of the interleukin 1 system in pregnancy and labor.Reproductive sciences (Thousand Oaks, Calif.) · 2014Observational
- Prostaglandins are essential for cervical ripening in LPS-mediated preterm birth but not term or antiprogestin-driven preterm ripening.Endocrinology · 2014Article
- Vaginal Misoprostol vs Vaginal Misoprostol With Estradiol for Labor Induction: A Prospective Double Blind Study.Journal of obstetrics and gynaecology of India · 2012Article
- Cervical tissue engineering using silk scaffolds and human cervical cells.Tissue engineering. Part A · 2010Article
- Article
- Interleukin-8 is involved in cervical dilatation but not in prelabour cervical ripening.Clinical and experimental immunology · 2004Article
- EP2/EP4 signalling inhibits monocyte chemoattractant protein-1 production induced by interleukin 1beta in synovial fibroblasts.Annals of the rheumatic diseases · 2004Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The aim of this study was to identify, in cultured human cervical fibroblasts, the mechanisms by which interleukin (IL)-1beta induces the synthesis of glycosaminoglycans (GAG) and to explore the putative role of prostaglandin E(2) (PGE(2)) in this process. Exposure of the cells for 24 h to IL-1beta induced a significant (P < 0.05) dose-dependent increase in GAG synthesis. IL-1beta (1 ng/ml) induced the expression of cyclooxygenase-2 (COX-2) protein 6 h after treatment, accompanied by a 7.5-fold increase in PGE(2) production. We confirmed that NS398, a selective COX-2 inhibitor, dose-dependently blocked PGE(2) augmentation following IL-1beta treatment. AH23848, the selective EP(4) receptor antagonist, completely abolished IL-1beta-induced GAG synthesis, whereas AH6809, an EP(2) receptor antagonist, had no effect on the stimulatory effects of IL-1beta. Furthermore, we demonstrated that 6 h exposure to IL-1beta induced a notable increase in EP(4) receptor mRNA expression and a decrease in EP(1) receptor mRNA but had no effect on the expression of EP(2) and EP(3) receptor transcripts. In conclusion, these findings indicate that IL-1beta not only induced GAG synthesis by increasing COX-2 protein expression and the subsequent PGE(2) production but also enhanced the responsiveness of cervical fibroblasts to PGE(2) by selectively up-regulating EP(4) receptor mRNA expression. These results suggest that PGE(2) may regulate human cervical ripening in an autocrine/paracrine manner via EP(4) receptors.
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