ArticleMolecular and cellular biology2003
Nonsense-containing mRNAs that accumulate in the absence of a functional nonsense-mediated mRNA decay pathway are destabilized rapidly upon its restitution.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Cytoplasmic mRNA decay and quality control machineries in eukaryotes.Nature reviews. Genetics · 2025Review
- Features and factors that dictate if terminating ribosomes cause or counteract nonsense-mediated mRNA decay.The Journal of biological chemistry · 2022Review
- Nuclear SR-protein mediated mRNA quality control is continued in cytoplasmic nonsense-mediated decay.RNA biology · 2021Article
- Unique Terminal Regions and Specific Deletions of the Segmented Double-Stranded RNA Genome of Alternaria Alternata Virus 1, in the Proposed Family Alternaviridae.Frontiers in microbiology · 2021Article
- UPF1-Mediated RNA Decay-Danse Macabre in a Cloud.Biomolecules · 2020Review
- Selective profiling of ribosomes associated with yeast Upf proteins.Methods (San Diego, Calif.) · 2019Article
- Nonsense-Mediated mRNA Decay Begins Where Translation Ends.Cold Spring Harbor perspectives in biology · 2019Review
- The Substrates of Nonsense-Mediated mRNA Decay inG3 (Bethesda, Md.) · 2018Article
- Processing of Potato Spindle Tuber Viroid RNAs in Yeast, a Nonconventional Host.Journal of virology · 2017Article
- NMD monitors translational fidelity 24/7.Current genetics · 2017Review
- High-resolution profiling of NMD targets in yeast reveals translational fidelity as a basis for substrate selection.RNA (New York, N.Y.) · 2017Article
- Nonsense-mediated mRNA decay: novel mechanistic insights and biological impact.Wiley interdisciplinary reviews. RNA · 2016Review
- Article
- Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain.RNA (New York, N.Y.) · 2015Article
- Review
- Regulation of natural mRNAs by the nonsense-mediated mRNA decay pathway.Eukaryotic cell · 2014Review
- In vivo determination of direct targets of the nonsense-mediated decay pathway in Drosophila.G3 (Bethesda, Md.) · 2014Article
- Cap-binding complex (CBC).The Biochemical journal · 2014Review
- Making sense of nonsense.Nature structural & molecular biology · 2013Article
- Nonsense-mediated mRNA decay occurs during eIF4F-dependent translation in human cells.Nature structural & molecular biology · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Nonsense-mediated mRNA decay (NMD) is a conserved proofreading mechanism that protects eukaryotic cells from the potentially deleterious effects of truncated proteins. Studies of Saccharomyces cerevisiae imply that NMD is a predominantly cytoplasmic decay pathway, while studies of mammalian systems suggest that decay of most substrate mRNAs may occur while they are still associated with the nucleus, possibly during a round of translation that occurs during their export to the cytoplasm. Complete entry of the latter mRNAs into the cytoplasm appears to render them immune to further NMD; i.e., they escape further susceptibility to this decay pathway. To determine if yeast cytoplasmic nonsense-containing mRNAs that evade decay are subsequently immune to NMD, we examined the consequences of placing each of the three UPF/NMD genes under the control of a galactose-inducible promoter. The decay kinetics of ADE2 and PGK1 nonsense-containing mRNAs were then analyzed when expression of UPF1, NMD2, or UPF3 was either repressed or subsequently induced. Results from these experiments demonstrated that activation of NMD caused rapid and immediate degradation of both substrate transcripts, with half-lives of both stable mRNA populations shortened to approximately 7 min. These findings make it unlikely that yeast nonsense-containing mRNAs can escape degradation by NMD and indicate that such mRNAs are available to this decay pathway at each round of translation.
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