ArticleMolecular and cellular biology2003
The dihydrofolate reductase origin of replication does not contain any nonredundant genetic elements required for origin activity.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- Quantity and quality of minichromosome maintenance protein complexes couple replication licensing to genome integrity.Communications biology · 2024Review
- Genome-wide mapping of human DNA replication by optical replication mapping supports a stochastic model of eukaryotic replication.Molecular cell · 2021Article
- Transcript availability dictates the balance between strand-asynchronous and strand-coupled mitochondrial DNA replication.Nucleic acids research · 2018Article
- Kaposi's Sarcoma-Associated Herpesvirus Deregulates Host Cellular Replication during Lytic Reactivation by Disrupting the MCM Complex through ORF59.Journal of virology · 2018Article
- How MCM loading and spreading specify eukaryotic DNA replication initiation sites.F1000Research · 2016Review
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Origin of DNA replication at the human lamin B2 locus: OBR or ABR?Cell cycle (Georgetown, Tex.) · 2012Article
- Regulation of DNA replication within the immunoglobulin heavy-chain locus during B cell commitment.PLoS biology · 2012Article
- Genome-scale analysis of metazoan replication origins reveals their organization in specific but flexible sites defined by conserved features.Genome research · 2011Article
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
- The dyad symmetry element of Epstein-Barr virus is a dominant but dispensable replication origin.PloS one · 2011Article
- Eukaryotic DNA replication origins: many choices for appropriate answers.Nature reviews. Molecular cell biology · 2010Review
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- [Establishment of spatial and temporal program for mammalian chromosome replication].Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme · 2009Review
- Multiple mechanisms contribute to Schizosaccharomyces pombe origin recognition complex-DNA interactions.The Journal of biological chemistry · 2008Article
- A revisionist replicon model for higher eukaryotic genomes.Journal of cellular biochemistry · 2008Review
- Essential elements of a licensed, mammalian plasmid origin of DNA synthesis.Molecular and cellular biology · 2006Article
- The Chinese hamster dihydrofolate reductase replication origin decision point follows activation of transcription and suppresses initiation of replication within transcription units.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Chinese hamster dihydrofolate reductase (DHFR) origin of replication consists of a broad zone of potential initiation sites scattered throughout a 55-kb intergenic spacer, with at least three sites being preferred (ori-beta, ori-beta', and ori-gamma). We previously showed that deletion of the most active site or region (ori-beta) has no demonstrable effect on initiation in the remainder of the intergenic spacer nor on the time of replication of the DHFR locus as a whole. In the present study, we have now deleted ori-beta', both ori-beta and ori-beta', an 11-kb region just downstream from the DHFR gene, or the central approximately 40-kb core of the spacer. The latter two deletions together encompass >95% of the initiation sites that are normally used in this locus. Two-dimensional gel analysis shows that initiation still occurs in the early S phase in the remainder of the intergenic spacer in each of these deletion variants. Even removal of the 40-kb core fails to elicit a significant effect on the time of replication of the DHFR locus in the S period; indeed, in the truncated spacer that remains, the efficiency of initiation actually appears to increase relative to the corresponding region in the wild-type locus. Thus, if replicators control the positions of nascent strand start sites in this complex origin, either (i) there must be a very large number of redundant elements in the spacer, each of which regulates initiation only in its immediate environment, or (ii) they must lie outside the central core in which the vast majority of nascent strand starts occur.
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