ArticleNucleic acids research2003
Fluoride-cleavable biotinylation phosphoramidite for 5'-end-labeling and affinity purification of synthetic oligonucleotides.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 45 citations in OpenAlex.
- Long oligos: direct chemical synthesis of genes with up to 1728 nucleotides.Chemical science · 2025Article
- De Novo Synthesis of Error-Free Long Oligos.Current protocols · 2024Article
- For Catching-by-Polymerization Oligo Purification: Scalable Synthesis of the Precursors to the Polymerizable Tagging Phosphoramidites.PeerJ organic chemistry · 2024Article
- Engineering Enzyme-Cleavable Oligonucleotides by Automated Solid-Phase Incorporation of Cathepsin B Sensitive Dipeptide Linkers.Angewandte Chemie (International ed. in English) · 2022Article
- Engineering Enzyme-Cleavable Oligonucleotides by Automated Solid-Phase Incorporation of Cathepsin B Sensitive Dipeptide Linkers.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- Parallel, Large-Scale, and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-Length Sequence by Polymerization Technique.Organic process research & development · 2018Article
- A High-Throughput Process for the Solid-Phase Purification of Synthetic DNA Sequences.Current protocols in nucleic acid chemistry · 2017Article
- Synthesis of Oligodeoxynucleotides Containing Electrophilic Groups.Organic letters · 2016Article
- Highly parallel oligonucleotide purification and functionalization using reversible chemistry.Nucleic acids research · 2012Article
- Linker phosphoramidite reagents for the attachment of the first nucleoside to underivatized solid-phase supports.Nucleic acids research · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5'-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5'-end of DNA. This linkage proved to be completely stable under certain post-synthetic DNA cleavage/deprotection conditions [0.05 M K(2)CO(3) in MeOH, room temperature, 24 h and MeNH(2) (approximately 40%)/NH(4)OH (approximately 29%), 1:1 v/v, 65 degrees C, 30 min] while it can be readily broken by fluoride ion, releasing unmodified DNA. To demonstrate the use of this DNA biotinylation method, we applied this method in affinity purification of synthetic DNA. As revealed by HPLC analysis, biotinylated full-length DNA can be efficiently attached to NeutrAvidin coated microspheres, and failure sequences can be readily removed. Subsequent treatment of the microspheres with pyridine/HF released high quality full-length unmodified DNA in good yield.
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Registered trials
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