ArticleNucleic acids research2003
Identification of a cis-acting element required for shunt-mediated translational initiation of the Sendai virus Y proteins.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Ribosomal control in RNA virus-infected cells.Frontiers in microbiology · 2022Review
- Non-Canonical Translation Initiation Mechanisms Employed by Eukaryotic Viral mRNAs.Biochemistry. Biokhimiia · 2021Review
- Article
- VIGOR extended to annotate genomes for additional 12 different viruses.Nucleic acids research · 2012Article
- RNA elements directing translation of the duck hepatitis B Virus polymerase via ribosomal shunting.Journal of virology · 2011Article
- Molecular dissection of the prototype foamy virus (PFV) RNA 5'-UTR identifies essential elements of a ribosomal shunt.Nucleic acids research · 2009Article
- Article
- Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus.RNA (New York, N.Y.) · 2006Article
- Tethering of eIF4G to adenoviral mRNAs by viral 100k protein drives ribosome shunting.Genes & development · 2004Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shunting is a mechanism that permits translational initiation at internal codons positioned in proximity to a ribosome acceptor sequence. Sendai virus exploits shunting to express a series of proteins that initiate at the fourth and fifth start sites on the P/C mRNA (namely, the Y1 and Y2 proteins, respectively). Shunt-mediated initiation at these sites is codon independent. In an attempt to characterise the acceptor site, an extensive deletion analysis was performed spanning the entire C ORF. Only mutants flanking the Y1/Y2 start sites exhibited altered shunt phenotypes. Some of these significantly enhanced shunting efficiency to the point where the Y1/Y2 proteins were the major translational products of the mRNA. Additionally, removal of a short region just downstream of the Y2 start codon (referred to as Delta10) ablated all Y protein initiation via shunting but had no effect on Y expression when the AUG codons were viewed by a scanning ribosome. Point mutations introduced into this Delta10 sequence severely perturbed shunt-mediated initiation. We also provide evidence that changes in this region of the P/C mRNA may be used to modulate Y protein expression levels in different viral strains.
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