ArticleThe Journal of physiology2003
Streptomycin and intracellular calcium modulate the response of single guinea-pig ventricular myocytes to axial stretch.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Calcium and arrhythmogenesis.Physiological reviews · 2007Pooled it
- Stretch-Induced Increase in CaJournal of the American Heart Association · 2025Article
- Mechanotransduction-Driven Modulation ofInternational journal of molecular sciences · 2025Article
- Review
- Identification of RNA reads encoding different channels in isolated rat ventricular myocytes and the effect of cell stretching on L-type CaBiology direct · 2023Article
- Differences in Mechanical, Electrical and Calcium Transient Performance of the Isolated Right Atrial and Ventricular Myocardium of Guinea Pigs at Different Preloads (Lengths).International journal of molecular sciences · 2023Article
- Enhancing iPSC-CM Maturation Using a Matrigel-Coated Micropatterned PDMS Substrate.Current protocols · 2022Article
- Mechano-electric and mechano-chemo-transduction in cardiomyocytes.The Journal of physiology · 2020Review
- Mechanically Induced Ectopy via Stretch-Activated Cation-Nonselective Channels Is Caused by Local Tissue Deformation and Results in Ventricular Fibrillation if Triggered on the Repolarization Wave Edge (Commotio Cordis).Circulation. Arrhythmia and electrophysiology · 2017Article
- Effect of electrical stimulation-induced muscle force and streptomycin treatment on muscle and trabecular bone mass in early-stage disuse musculoskeletal atrophy.Journal of musculoskeletal & neuronal interactions · 2015Article
- Increased cell membrane capacitance is the dominant mechanism of stretch-dependent conduction slowing in the rabbit heart: a computational study.Cellular and molecular bioengineering · 2015Article
- Arrhythmogenic effects by local left ventricular stretch: effects of flecainide and streptomycin.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- Streptomycin inhibits electrophysiological changes induced by stretching of chronically infarcted rat hearts.Journal of Zhejiang University. Science. B · 2014Article
- Role of autocrine/paracrine mechanisms in response to myocardial strain.Pflugers Archiv : European journal of physiology · 2011Review
- Mechano-electric feedback in the fish heart.PloS one · 2010Article
- Stretch-activated channel activation promotes early afterdepolarizations in rat ventricular myocytes under oxidative stress.American journal of physiology. Heart and circulatory physiology · 2009Article
- The relevance of non-excitable cells for cardiac pacemaker function.The Journal of physiology · 2007Article
- Stimulus interval, rate and direction differentially regulate phosphorylation for mechanotransduction in neonatal cardiac myocytes.FEBS letters · 2007Article
- A mathematical model of the slow force response to stretch in rat ventricular myocytes.Biophysical journal · 2007Article
- Activation of Na+-H+ exchange and stretch-activated channels underlies the slow inotropic response to stretch in myocytes and muscle from the rat heart.The Journal of physiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We tested the hypothesis that both stretch-activated channels (SACs) and intracellular calcium ([Ca(2+)](i)) are important in the electrical response of single guinea-pig ventricular myocytes to axial stretch. Myocytes were attached to carbon fibre transducers and stretched, sarcomere length increased by approximately 9 %, and there was a prolongation of the action potential duration. Streptomycin, a blocker of SACs, had no effect upon the shortening, [Ca(2+)](i) transients or action potentials of electrically stimulated, unstretched myocytes, at a concentration of 50 microM, but at 40 microM, prevented any stretch-induced increase in action potential duration. Under action potential clamp, stretch elicited a current with a linear current-voltage relationship that was inward at membrane potentials negative to its reversal potential of -30 mV, in 10 of 24 cells tested, and was consistent with the activation of non-specific, cationic SACs. This current was not seen in any stretched cells that were exposed to 40 microM streptomycin. However, exposure of cells to 5 microM BAPTA-AM, in order to reduce [Ca(2+)](i) transients, also abolished stretch-induced prolongation of the action potential. We conclude that both SACs and [Ca(2+)](i) are important in the electrical response of cardiac myocytes to stretch, and propose that stretch-induced changes in electrical activity and [Ca(2+)](i) may be linked by inter-dependent mechanisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.