ArticleJournal of virology2003
Replication of Carnation Italian ringspot virus defective interfering RNA in Saccharomyces cerevisiae.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 62 citations in OpenAlex.
- Carnation Italian Ringspot Virus p36 Expression Induces Mitochondrial Fission and Respiratory Chain Complex Impairment in Yeast.International journal of molecular sciences · 2023Article
- The intriguing phenomenon of cross-kingdom infections of plant and insect viruses to fungi: Can other animal viruses also cross-infect fungi?PLoS pathogens · 2023Article
- Exogenous and endogenous dsRNAs perceived by plant Dicer-like 4 protein in the RNAi-depleted cellular context.Cellular & molecular biology letters · 2023Article
- Impediometric Electrochemical Sensor Based on The Inspiration of Carnation Italian Ringspot Virus Structure to Detect an Attommolar of miR.Scientific reports · 2020Article
- Full-Length Hairpin RNA Accumulates at High Levels in Yeast but Not in Bacteria and Plants.Genes · 2019Article
- Identification of aViruses · 2018Article
- Susceptibility Genes to Plant Viruses.Viruses · 2018Review
- Yeast for virus research.Microbial cell (Graz, Austria) · 2017Review
- Homeologs of the Nicotiana benthamiana Antiviral ARGONAUTE1 Show Different Susceptibilities to microRNA168-Mediated Control.Plant physiology · 2015Article
- Yeast and the AIDS virus: the odd couple.Journal of biomedicine & biotechnology · 2012Review
- Dissecting virus-plant interactions through proteomics approaches.Current proteomics · 2010Article
- Article
- An inhibitory interaction between viral and cellular proteins underlies the resistance of tomato to nonadapted tobamoviruses.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Saccharomyces cerevisiae: a versatile eukaryotic system in virology.Microbial cell factories · 2007Article
- Inducible yeast system for Viral RNA recombination reveals requirement for an RNA replication signal on both parental RNAs.Journal of virology · 2006Article
- Saccharomyces cerevisiae: a useful model host to study fundamental biology of viral replication.Virus research · 2006Review
- Proteomics analysis of the tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication.Journal of virology · 2006Article
- Role of an internal and two 3'-terminal RNA elements in assembly of tombusvirus replicase.Journal of virology · 2005Article
- Genome-wide screen identifies host genes affecting viral RNA recombination.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two plasmids from which the sequences coding for the 36- and 95-kDa proteins of Carnation Italian ringspot virus (CIRV) could be transcribed in vivo in the yeast Saccharomyces cerevisiae under the control of the ADH1 promoter and terminator were constructed. The two proteins, which constitute the viral replicase, were correctly translated and integrated into membranes of the yeast cells. An additional plasmid was introduced in yeasts expressing the CIRV replicase, from which a defective interfering (DI) RNA (DI-7 RNA) could be transcribed under the control of the GAL1 promoter and terminated by the Tobacco ringspot virus satellite ribozyme, which cleaved 19 nucleotides downstream of the 3' end of DI RNA. The DI-7 RNA transcripts were amplified by the viral replicase as demonstrated by the restoration of the authentic 3' end, the requirement of a specific cis-acting signal at this terminus, the preferential accumulation of molecules with the authentic 5' terminus (AGAAA), the synthesis of head-to-tail dimers, the presence of negative strands, and the incorporation of 5-bromo-UTP. Additionally, transformation with a dimeric construct of DI-7 RNA led to the synthesis of monomers, mimicking the activity of the viral replicase in plant cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.