ArticleJournal of virology2003
A mutant form of the tax protein of bovine leukemia virus (BLV), with enhanced transactivation activity, increases expression and propagation of BLV in vitro but not in vivo.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Bovine Leukemia Virus and Human Breast Cancer: A Review of Clinical and Molecular Evidence.Viruses · 2025Review
- Phylogenetic analysis of the partial sequences of theVirusdisease · 2023Article
- The bovine leukemia virus-derived long non-coding RNA AS1-S binds to bovine hnRNPM and alters the interaction between hnRNPM and host mRNAs.Microbiology spectrum · 2023Article
- Correlation between the Biodistribution of Bovine Leukemia Virus in the Organs and the Proviral Load in the Peripheral Blood during Early Stages of Experimentally Infected Cattle.Pathogens (Basel, Switzerland) · 2023Article
- Article
- Effects of Naturally Occurring Mutations in Bovine Leukemia Virus 5'-LTR and Tax Gene on Viral Transcriptional Activity.Pathogens (Basel, Switzerland) · 2020Article
- Regulation of Expression and Latency in BLV and HTLV.Viruses · 2020Review
- Vector control efficacy of fly nets on preventing bovine leukemia virus transmission.The Journal of veterinary medical science · 2018Article
- Cooperation of PD-1 and LAG-3 in the exhaustion of CD4Veterinary research · 2018Article
- Molecular epidemiological survey and genetic characterization of ovine gammaherpesvirus-2 in Mongolian livestock.The Journal of veterinary medical science · 2017Article
- Epidemiology and genetic diversity of bovine leukemia virus.Virology journal · 2017Review
- A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis.Retrovirology · 2016Article
- Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle.Retrovirology · 2015Article
- Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR.BMC veterinary research · 2013Article
- Mechanisms of pathogenesis induced by bovine leukemia virus as a model for human T-cell leukemia virus.Frontiers in microbiology · 2013Review
- BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status.BMC veterinary research · 2012Article
- Article
- Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade.Veterinary research · 2011Article
- BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm.Retrovirology · 2010Article
- A dose-effect relationship for deltaretrovirus-dependent leukemogenesis in sheep.Retrovirology · 2009Article
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a previous study, we identified an interesting mutant form of the Tax protein of bovine leukemia virus (BLV), designated D247G. This mutant protein strongly transactivated the long terminal repeat of BLV and was also able to transactivate the cellular proto-oncogene c-fos. This finding suggested that BLV that encode the mutant protein might propagate and induce lymphoma more efficiently than wild-type BLV. To characterize the effects of the strong transactivation activity of the mutant Tax protein, we constructed an infectious molecular clone of BLV that encoded D247G and examined the replication and propagation of the virus in vitro and in vivo. Cultured cells were transfected with the wild-type and mutant BLV, and then levels of viral proteins and particles and the propagation of viruses were compared. As expected, in vitro, mutant BLV produced more viral proteins and particles and was transmitted very effectively. We injected the wild-type and mutant BLV into sheep, which are easily infected with BLV, and monitored the proportion of BLV-positive cells in the blood and the expression of BLV RNA for 28 weeks. By contrast to the results of our analyses in vitro, we found no significant difference in the viral load or the expression of viral RNA between sheep inoculated with wild-type or mutant BLV. Our observations indicate that the mutant D247G Tax protein does not enhance the expansion of BLV and that there might be a dominant mechanism for regulation of the expression of BLV in vivo.
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