ArticleJournal of virology2003
Alphavirus minus-strand synthesis and persistence in mouse embryo fibroblasts derived from mice lacking RNase L and protein kinase R.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice.Nature microbiology · 2026Article
- Hidden in Plain Sight: Alphavirus Persistence and Its Potential for Driving Chronic Pathogenesis.Viruses · 2025Review
- Mouse Adenovirus Type 1 E4orf6 Induces PKR Degradation.Journal of virology · 2022Article
- Host Defence RNases as Antiviral Agents against Enveloped Single Stranded RNA Viruses.Virulence · 2021Review
- Article
- ADP-ribosyl-binding and hydrolase activities of the alphavirus nsP3 macrodomain are critical for initiation of virus replication.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Virus-derived small RNAs: molecular footprints of host-pathogen interactions.Wiley interdisciplinary reviews. RNA · 2016Review
- Alphavirus RNA synthesis and non-structural protein functions.The Journal of general virology · 2015Review
- Article
- Template role of double-stranded RNA in tombusvirus replication.Journal of virology · 2014Article
- Article
- Requirement for the amino-terminal domain of sindbis virus nsP4 during virus infection.Journal of virology · 2011Article
- Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling.Journal of virology · 2010Article
- Respiratory syncytial virus limits alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) phosphorylation to maintain translation and viral replication.The Journal of biological chemistry · 2010Article
- hnRNP A1 interacts with the genomic and subgenomic RNA promoters of Sindbis virus and is required for the synthesis of G and SG RNA.Journal of biomedical science · 2010Article
- Fate of minus-strand templates and replication complexes produced by a p23-cleavage-defective mutant of Sindbis virus.Journal of virology · 2009Article
- PKR acts early in infection to suppress Semliki Forest virus production and strongly enhances the type I interferon response.The Journal of general virology · 2009Article
- Characterization of purified Sindbis virus nsP4 RNA-dependent RNA polymerase activity in vitro.Virology · 2009Article
- Role for conserved residues of sindbis virus nonstructural protein 2 methyltransferase-like domain in regulation of minus-strand synthesis and development of cytopathic infection.Journal of virology · 2008Article
- A new role for ns polyprotein cleavage in Sindbis virus replication.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We report our studies to probe the possible role of the host response to double-stranded RNA in cessation of alphavirus minus-strand synthesis. Mouse embryo fibroblasts (MEF) from Mx1-deficient mice that also lack either the protein kinase R (PKR) or the latent RNase L or both PKR and RNase L were screened. In RNase L-deficient but not wild-type or PKR-deficient MEF, there was continuous synthesis of minus-strand templates and the formation of new replication complexes producing viral plus strands. Inhibiting translation caused minus-strand synthesis to stop and a loss of transcription activity of the mature replication complexes. This turnover of replication complexes that were stable in cells containing RNase L suggested that RNase L plays some role, albeit possibly indirect, in the formation of stable replication complexes during alphavirus infection. In addition, confluent monolayers of RNase L-deficient murine cells readily established persistent infections and were not killed. This phenotype is contrary to what has been observed for infection in vertebrate cells with a presumably functional RNase L gene and more resembled alphavirus replication in Aedes mosquito cells, in which the activity of replication complexes making plus stands was also found to decay with inhibition of translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.