ArticleEMBO reports2003
Cyclic AMP is sufficient for triggering the exocytic recruitment of aquaporin-2 in renal epithelial cells.
Article in EMBO reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 75 citations in OpenAlex.
- Clinical Potential of Misshapen/NIKs-Related Kinase (MINK) 1-A Many-Sided Element of Cell Physiology and Pathology.Current issues in molecular biology · 2024Review
- Expression Regulation and Trafficking of Aquaporins.Advances in experimental medicine and biology · 2023Review
- The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal.NPJ biofilms and microbiomes · 2022Article
- The Role of Apelin/Apelin Receptor in Energy Metabolism and Water Homeostasis: A Comprehensive Narrative Review.Frontiers in physiology · 2021Review
- The physiological and molecular mechanisms to maintain water and salt homeostasis in response to high salt intake in Mongolian gerbils (Meriones unguiculatus).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2020Article
- Article
- Defective Store-Operated Calcium Entry Causes Partial Nephrogenic Diabetes Insipidus.Journal of the American Society of Nephrology : JASN · 2016Article
- Signaling Modification by GPCR Heteromer and Its Implication on X-Linked Nephrogenic Diabetes Insipidus.PloS one · 2016Article
- Dynamic regulation and dysregulation of the water channel aquaporin-2: a common cause of and promising therapeutic target for water balance disorders.Clinical and experimental nephrology · 2014Review
- Culturing primary rat inner medullary collecting duct cells.Journal of visualized experiments : JoVE · 2013Article
- Mechanism for targeting the A-kinase anchoring protein AKAP18δ to the membrane.The Journal of biological chemistry · 2012Article
- Aquaporin 2 promotes cell migration and epithelial morphogenesis.Journal of the American Society of Nephrology : JASN · 2012Article
- Monitoring single-channel water permeability in polarized cells.The Journal of biological chemistry · 2011Article
- Cytokine-induced paracrystals prolong the activity of signal transducers and activators of transcription (STAT) and provide a model for the regulation of protein solubility by small ubiquitin-like modifier (SUMO).The Journal of biological chemistry · 2011Article
- Mechanisms of vasopressin-induced intracellular Ca2+ oscillations in rat inner medullary collecting duct.American journal of physiology. Renal physiology · 2011Article
- Routes of epithelial water flow: aquaporins versus cotransporters.Biophysical journal · 2010Article
- Reciprocal regulation of aquaporin-2 abundance and degradation by protein kinase A and p38-MAP kinase.Journal of the American Society of Nephrology : JASN · 2010Article
- Aquaporins in kidney pathophysiology.Nature reviews. Nephrology · 2010Review
- A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2.American journal of physiology. Cell physiology · 2008Article
- Physiology and pathophysiology of the vasopressin-regulated renal water reabsorption.Pflugers Archiv : European journal of physiology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initial response of renal epithelial cells to the antidiuretic hormone arginine vasopressin (AVP) is an increase in cyclic AMP. By applying immunofluorescence, cell membrane capacitance and transepithelial water flux measurements we show that cAMP alone is sufficient to elicit the antidiuretic cellular response in primary cultured epithelial cells from renal inner medulla, namely the transport of aquaporin-2 (AQP2)-bearing vesicles to, and their subsequent fusion with, the plasma membrane (AQP2 shuttle). The AQP2 shuttle is evoked neither by AVP-independent Ca(2+) increases nor by AVP-induced Ca(2+) increases. However, clamping cytosolic Ca(2+) concentrations below resting levels at 25 nM inhibited exocytosis. Exocytosis was confined to a slow monophasic response, and readily releasable vesicles were missing. Analysis of endocytic capacitance steps revealed that cAMP does not decelerate the retrieval of AQP2 from the plasma membrane. Our data suggest that cAMP initiates an early step, namely the transport of AQP2-bearing vesicles towards the plasma membrane, and do not support a regulatory function for Ca(2+) in the AQP2 shuttle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.