ArticleBiophysical journal2003
Vibrational spectroscopy of an algal Phot-LOV1 domain probes the molecular changes associated with blue-light reception.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- QM/MM Calculated Triplet-Singlet Infrared Difference Spectra of LOV2 Photoreceptor Domains and FMN in Solution.ACS omega · 2026Article
- Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.Journal of the American Chemical Society · 2025Article
- Article
- QM calculations predict the energetics and infrared spectra of transient glutamine isomers in LOV photoreceptors.Physical chemistry chemical physics : PCCP · 2021Article
- Peripheral Methionine Residues Impact Flavin Photoreduction and Protonation in an Engineered LOV Domain Light Sensor.Biochemistry · 2021Article
- In-cell infrared difference spectroscopy of LOV photoreceptors reveals structural responses to light altered in living cells.The Journal of biological chemistry · 2020Article
- Modulation of the flavin-protein interactions in NADH peroxidase and mercuric ion reductase: a resonance Raman study.European biophysics journal : EBJ · 2018Article
- Aureochrome 1 illuminated: structural changes of a transcription factor probed by molecular spectroscopy.PloS one · 2014Article
- Signals of LOV1: a computer simulation study on the wildtype LOV1-domain of Chlamydomonas reinhardtii and its mutants.Journal of molecular modeling · 2012Article
- Specific effects of reactive thiol drugs on mitochondrial bioenergetics.Journal of bioenergetics and biomembranes · 2011Review
- Light-induced structural changes of the LOV2 domains in various phototropins revealed by FTIR spectroscopy.Biophysics (Nagoya-shi, Japan) · 2011Review
- Key dynamics of conserved asparagine in a cryptochrome/photolyase family protein by fourier transform infrared spectroscopy.Biochemistry · 2010Article
- Conformational heterogeneity and propagation of structural changes in the LOV2/Jalpha domain from Avena sativa phototropin 1 as recorded by temperature-dependent FTIR spectroscopy.Biophysical journal · 2009Article
- Primary reactions of the LOV2 domain of phototropin studied with ultrafast mid-infrared spectroscopy and quantum chemistry.Biophysical journal · 2009Article
- Light signal transduction pathway from flavin chromophore to the J alpha helix of Arabidopsis phototropin1.Biophysical journal · 2009Article
- Time-resolved Fourier transform infrared study on photoadduct formation and secondary structural changes within the phototropin LOV domain.Biophysical journal · 2009Article
- Hydrogen bond switching among flavin and amino acid side chains in the BLUF photoreceptor observed by ultrafast infrared spectroscopy.Biophysical journal · 2008Article
- When light falls in LOV: a quantum mechanical/molecular mechanical study of photoexcitation in Phot-LOV1 of Chlamydomonas reinhardtii.The journal of physical chemistry. B · 2005Article
- The phot LOV2 domain and its interaction with LOV1.Biophysical journal · 2005Article
- Plant blue-light receptors.Planta · 2005Review
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3 authors.
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Abstract
The LOV1 domain of the blue light Phot1-receptor (phototropin homolog) from Chlamydomonas reinhardtii has been studied by vibrational spectroscopy. The FMN modes of the dark state of LOV1 were identified by preresonance Raman spectroscopy and assigned to molecular vibrations. By comparing the blue-light-induced FTIR difference spectrum with the preresonance Raman spectrum, most of the differences are due to FMN modes. Thus, we exclude large backbone changes of the protein that might occur during the phototransformation of the dark state LOV1-447 into the putative signaling state LOV1-390. Still, the presence of smaller amide difference bands cannot be excluded but may be masked by overlapping FMN modes. The band at 2567 cm(-1) is assigned to the S-H stretching vibration of C57, the residue that forms the transient thio-adduct with the chromophore FMN. The occurrence of this band is evidence that C57 is protonated in the dark state of LOV1. This result challenges conclusions from the homologous LOV2 domain from oat that the thiolate of the corresponding cysteine is the reactive species.
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