ArticleClinical and diagnostic laboratory immunology2003
Evaluation of four commercially available Epstein-Barr virus enzyme immunoassays with an immunofluorescence assay as the reference method.
Article in Clinical and diagnostic laboratory immunology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 71 citations in OpenAlex.
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- Status of Epstein-Barr Virus Coinfection withJournal of oncology · 2017Review
- Prevalence and molecular profiling of Epstein Barr virus (EBV) among healthy blood donors from different nationalities in Qatar.PloS one · 2017Article
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- Correlates of illness severity in infectious mononucleosis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- Performance of the architect EBV antibody panel for determination of Epstein-Barr virus infection stage in immunocompetent adolescents and young adults with clinical suspicion of infectious mononucleosis.Clinical and vaccine immunology : CVI · 2014Article
- Evaluation of the Architect Epstein-Barr Virus (EBV) viral capsid antigen (VCA) IgG, VCA IgM, and EBV nuclear antigen 1 IgG chemiluminescent immunoassays for detection of EBV antibodies and categorization of EBV infection status using immunofluorescence assays as the reference method.Clinical and vaccine immunology : CVI · 2014Article
- Performance of two commercially available automated immunoassays for the determination of Epstein-Barr virus serological status.Clinical and vaccine immunology : CVI · 2012Article
- Serological diagnosis of Epstein-Barr virus infection: Problems and solutions.World journal of virology · 2012Review
- Performance of new enzyme-linked fluorescent assays for detection of Epstein-Barr virus specific antibodies in routine diagnostics.Wiener klinische Wochenschrift · 2011Article
- Mannose-binding lectin genotypes and susceptibility to epstein-barr virus infection in infancy.Clinical and vaccine immunology : CVI · 2010Article
- Detection of Viral Citrullinated Peptide Antibodies Directed Against EBV or VCP: In Early Rheumatoid Arthritis Patients of Indian Origin.Journal of laboratory physicians · 2010Article
- Comparison of three automated immunoassay methods for the determination of Epstein-Barr virus-specific immunoglobulin M.Clinical and vaccine immunology : CVI · 2010Article
- Stress-related Epstein-Barr virus reactivation.Clinical and experimental medicine · 2010Article
- Search for Anti-EA(D) Antibodies in Subjects with an "Isolated VCA IgG" Pattern.International journal of microbiology · 2010Article
- Epstein-Barr virus antibody level and gastric cancer risk in Korea: a nested case-control study.British journal of cancer · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four commercially available enzyme immunoassays (EIAs) (Novitec, Biotest, Virotech, and DiaSorin) were evaluated, with an indirect immunofluorescence assay as the reference method, for Epstein-Barr virus (EBV) VCA (viral capsid antigen) immunoglobulin G (IgG), VCA IgM, or EBNA (EBV nuclear antigen) IgG at three different locations (Homburg, Stuttgart, and Dresden). Serum samples from 66 immunocompetent patients with infectious mononucleosis, 73 patients without prior EBV infection, and 96 patients with past EBV infections and 29 serum samples with possible cross-reactions to other herpesviruses were included. In addition, 25 samples from an extensively pretested panel that is commercially available (Boston Biomedica) were tested. Each sample was tested at only one location. The four EIAs varied considerably in performance. When analyzing for EBV diagnosis, the Novitec assay performed the best, with 4.9% discrepant diagnoses, followed by the Biotest, Virotech, and DiaSorin assays, with 6.8, 11.7, and 14.0% discrepant diagnoses, respectively. On the basis of single-parameter analysis, the Novitec assay also showed the lowest number of discrepant results, with 3.5%, compared with the Virotech, Biotest, and DiaSorin assays, which produced 5.4, 6.4, and 8.6% discrepant results, respectively. VCA assays using affinity-purified native antigens performed better than assays with recombinant or synthetic antigens. The synthetic EBNA-1s showed the lowest concordance with the reference compared to recombinant p72. Commercially available EBV EIAs differed considerably in performance; however, some proved to be reliable and convenient alternatives to the indirect immunofluorescence assay for routine diagnostics. Native antigens, rather than synthetic peptides, are favored for EBV serology testing.
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