ArticleClinical and diagnostic laboratory immunology2003
Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle.
Article in Clinical and diagnostic laboratory immunology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Development and validation of a recombinant Rap1-based lateral flow immunoassay for rapid serodiagnosis of bovine babesiosis in Kazakhstan.Veterinary world · 2025Article
- Nilgai antelope display no signs of infection upon experimental challenge with a virulent Babesia bovis strain.Parasites & vectors · 2024Article
- A conserved motif in the immune-subdominant RAP-1 related antigen ofFrontiers in immunology · 2024Article
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- Vaccination with anFrontiers in immunology · 2023Article
- Thrombospondin-Related Anonymous Protein (TRAP) Family Expression byMicroorganisms · 2022Article
- A Culture-Adapted Strain ofFrontiers in cellular and infection microbiology · 2022Article
- Identification of novel immune correlates of protection against acute bovine babesiosis by superinfecting cattle withFrontiers in immunology · 2022Article
- Assessment of Babesia bovis 6cys A and 6cys B as components of transmission blocking vaccines for babesiosis.Parasites & vectors · 2021Article
- Validation of a multiplex PCR assay to detectJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Diagnostic Tools for the Identification ofPathogens (Basel, Switzerland) · 2019Review
- A novel modified-indirect ELISA based on spherical body protein 4 for detecting antibody during acute and long-term infections with diverse Babesia bovis strains.Parasites & vectors · 2017Article
- Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt.Parasites & vectors · 2015Article
- Validation and field evaluation of a competitive enzyme-linked immunosorbent assay for diagnosis of Babesia bovis infections in Argentina.Clinical and vaccine immunology : CVI · 2012Article
- Current advances in detection and treatment of babesiosis.Current medicinal chemistry · 2012Review
- Spherical body protein 4 is a new serological antigen for global detection of Babesia bovis infection in cattle.Clinical and vaccine immunology : CVI · 2011Article
- Comparison of indirect fluorescent antibody test (IFAT) and slide enzyme linked immunosorbent assay (SELISA) for diagnosis of Babesia bigemina infection in bovines.Tropical animal health and production · 2009Article
- Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle.Clinical and vaccine immunology : CVI · 2008Article
- Validation of a competitive enzyme-linked immunosorbent assay for detection of antibodies against Babesia bovis.Clinical and vaccine immunology : CVI · 2006Article
- Improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for detection of specific antibodies.Journal of clinical microbiology · 2004Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The competitive enzyme-linked immunosorbent assay (cELISA) format has proven to be an accurate, reliable, easily standardized, and high-throughput method for detecting hemoparasite infections. In the present study, a species-specific, broadly conserved, and tandemly repeated B-cell epitope within the C terminus of the rhoptry-associated protein 1 of the hemoparasite Babesia bovis was cloned and expressed as a histidine-tagged thioredoxin fusion peptide and used as antigen in a cELISA. The assay was optimized with defined negative and positive bovine sera, where positive sera inhibited the binding of the epitope-specific monoclonal antibody BABB75A4. The cELISA accurately differentiated animals with B. bovis-specific antibodies from uninfected animals and from animals with antibodies against other tick-borne hemoparasites (98.7% specificity). In addition, B. bovis-specific sera from Australia, Argentina, Bolivia, Puerto Rico, and Morocco inhibited the binding of BABB75A4, confirming conservation of the epitope. The assay first detected experimentally infected animals between 13 and 17 days postinfection, and with sera from naturally infected carrier cattle, was comparable to indirect immunofluorescence (98.3% concordance). The assay appears to have the characteristics necessary for an epidemiologic and disease surveillance tool.
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