ArticleThe Biochemical journal2003
Converging signals synergistically activate the LAMC2 promoter and lead to accumulation of the laminin gamma 2 chain in human colon carcinoma cells.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- An AI-assisted morphoproteomic approach is a supportive tool in esophagitis-related precision medicine.EMBO molecular medicine · 2025Article
- LncRNAs-Regulated High Expression of LAMC2 Reveals a Prognostic and Immunological Value in Pancreatic Adenocarcinoma.Biochemical genetics · 2024Article
- Anti-Laminin 332-Type Mucous Membrane Pemphigoid.Biomolecules · 2022Review
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- Urinary laminin-γ2 is a novel biomarker of non-muscle invasive urothelial carcinoma.Cancer science · 2015Article
- Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production.Fertility and sterility · 2013Article
- Expression of laminin 5-γ2 chain in cutaneous squamous cell carcinoma and its role in tumour invasion.British journal of cancer · 2011Article
- Uncoupled responses of Smad4-deficient cancer cells to TNFalpha result in secretion of monomeric laminin-gamma2.Molecular cancer · 2010Article
- Gene expression profiling identifies genes predictive of oral squamous cell carcinoma.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2008Article
- Divergent mechanisms underlie Smad4-mediated positive regulation of the three genes encoding the basement membrane component laminin-332 (laminin-5).BMC cancer · 2008Article
- Laminin-332-integrin interaction: a target for cancer therapy?Current medicinal chemistry · 2008Review
- The biological relevance of laminin 5gamma2 expression at the invading edge of colonic carcinomas.International journal of clinical and experimental pathology · 2008Article
- Article
- Migratory marker expression in fibroblast foci of idiopathic pulmonary fibrosis.Respiratory research · 2006Article
- Novel genes implicated in embryonal, alveolar, and pleomorphic rhabdomyosarcoma: a cytogenetic and molecular analysis of primary tumors.Neoplasia (New York, N.Y.) · 2006Article
- Kruppel-like factors regulate the Lama1 gene encoding the laminin alpha1 chain.The Journal of biological chemistry · 2004Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The trimeric extracellular matrix molecule laminin-5 and its constituent chains (alpha 3, beta 3, gamma 2) are normally not detectable intracellularly in intestinal epithelial cells but the laminin gamma 2 chain can be detected in cancer cells at the invasive front of a subset of colon carcinomas. These cells are subjected to cytokines such as transforming growth factor beta 1 (TGF-beta 1) and hepatocyte growth factor (HGF), produced by the tumour cells or by the surrounding stromal cells. The purpose of the present work was to investigate whether TGF-beta 1 and HGF, known to stimulate the LAMC2 gene encoding the laminin gamma 2 chain, might synergize to activate the LAMC2 promoter, and to identify the promoter elements involved. We find evidence for synergy between TGF-beta and HGF with respect to laminin gamma 2 chain expression and promoter activation and demonstrate that this requires the 5' activator protein-1 (AP-1) element of the promoter and an additional upstream element which is also responsive to co-expression of the Smad3 protein from the TGF-beta signalling pathway. The transcripts encoding the other laminin-5 chains are not synergistically activated by HGF and TGF-beta. Thus the synergistic activation of the LAMC2 gene is mediated via different cis-elements and results in an overproduction of the laminin gamma 2 chain relative to the other laminin-5 constituent chains. This difference may explain why laminin gamma 2 chains accumulate in the cells at the invasive front of colon carcinomas.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.