ArticleJournal of clinical microbiology2003
Identification of Candida spp. by randomly amplified polymorphic DNA analysis and differentiation between Candida albicans and Candida dubliniensis by direct PCR methods.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Genetic diversity and antifungal resistance in Candida albicans from VVC cases in Indian women.BMC microbiology · 2025Article
- Discrimination of Clinical and Food-DerivedPathogens (Basel, Switzerland) · 2025Article
- Categorizing and characterizing intestinal dysbiosis: evaluating stool microbial test clinical utility.Frontiers in microbiomes · 2025Article
- Article
- Antifungal and antibiofilm effects of probioticFrontiers in cellular and infection microbiology · 2024Article
- Phylogeny, evolution, and potential ecological relationship of cytochrome CYP52 enzymes in Saccharomycetales yeasts.Scientific reports · 2020Article
- Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present.Microorganisms · 2019Review
- Effective inactivation of Saccharomyces cerevisiae in minimally processed Makgeolli using low-pressure homogenization-based pasteurization.SpringerPlus · 2015Article
- In vivo and in vitro acquisition of resistance to voriconazole by Candida krusei.Antimicrobial agents and chemotherapy · 2014Article
- Rapid emergence of echinocandin resistance in Candida glabrata resulting in clinical and microbiologic failure.Antimicrobial agents and chemotherapy · 2013Article
- Candida species lens abscesses in infants with a history of neonatal Candida sepsis.Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus · 2013Article
- FKS2 mutations associated with decreased echinocandin susceptibility of Candida glabrata following anidulafungin therapy.Antimicrobial agents and chemotherapy · 2011Article
- Loss of in vitro resistance in Candida glabrata following discontinuation of fluconazole prophylaxis in a hematopoietic stem cell transplantation patient.Medical mycology · 2010Article
- Development of caspofungin resistance following prolonged therapy for invasive candidiasis secondary to Candida glabrata infection.Antimicrobial agents and chemotherapy · 2008Article
- Candida nivariensis isolated from an Indonesian human immunodeficiency virus-infected patient suffering from oropharyngeal candidiasis.Journal of clinical microbiology · 2008Article
- Molecular identification of unusual pathogenic yeast isolates by large ribosomal subunit gene sequencing: 2 years of experience at the United kingdom mycology reference laboratory.Journal of clinical microbiology · 2007Article
- Detection of Candida dubliniensis in Venezuela.Mycopathologia · 2005Article
- Discrimination between Candida albicans and Candida dubliniensis isolated from HIV-positive patients by using commercial method in comparison with PCR assay.Folia microbiologica · 2004Article
- Genetic diversity among clinical isolates of Candida glabrata analyzed by randomly amplified polymorphic DNA and multilocus enzyme electrophoresis analyses.Journal of clinical microbiology · 2003Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Because Candida species have innately highly variable antifungal susceptibilities, the availability of a fast and reliable species identification test is very important so that suitable and effective therapeutic measures may be taken. Using three oligonucleotide primers, we established a randomly amplified polymorphic DNA (RAPD) analysis method that enabled direct identification of the most common opportunistic pathogenic Candida species. RAPD analysis revealed a characteristic molecular fingerprint for each Candida species. Differences between the profiles for Candida albicans and C. dubliniensis were evident. RAPD analysis is a relatively easy, reproducible, and reliable technique that can be useful in providing genetic fingerprints for the identification of strains. In addition, a collection of different C. albicans strains was identified by a specific PCR based on multiple secreted aspartic proteinase (SAP) genes and the dipeptidyl aminopeptidase (DAP2) gene. Our findings demonstrate that PCR based upon the SAP and DAP2 sequences is a simple, rapid, clear, and direct technique for the identification and differentiation of C. albicans and C. dubliniensis.
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