ArticleJournal of clinical microbiology2003
Characterization of Mycobacterium tuberculosis complex DNAs from Egyptian mummies by spoligotyping.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
- Assessing the gender responsiveness of tuberculosis interventions: a scoping review (2000-2025).Systematic reviews · 2026Article
- Historical and Social Considerations upon Tuberculosis.Journal of preventive medicine and hygiene · 2025Article
- Respiratory Isolation for Tuberculosis: A Historical Perspective.The Journal of infectious diseases · 2025Article
- Molecular typing of Mycobacterium tuberculosis: a review of current methods, databases, softwares, and analytical tools.FEMS microbiology reviews · 2025Review
- The Egypt Genome Project.Nature genetics · 2024Article
- Evolution of orthopaedic diseases through four thousand three hundred years: from ancient Egypt with virtual examinations of mummies to the twenty-first century.International orthopaedics · 2024Article
- Nitrobenzoates and Nitrothiobenzoates with Activity againstMicroorganisms · 2023Article
- The Evolution of Diagnostic Techniques in the Paleopathology of Tuberculosis: A Scoping Review.Pathogens & immunity · 2023Article
- Mapping Research Trends of Medications for Multidrug-Resistant Pulmonary Tuberculosis Based on the Co-Occurrence of Specific Semantic Types in the MeSH Tree: A Bibliometric and Visualization-Based Analysis of PubMed Literature (1966-2020).Drug design, development and therapy · 2023Review
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- Chemical Synthesis of Cell Wall Constituents ofChemical reviews · 2021Review
- Ending TB: the world's oldest pandemic.Journal of the International AIDS Society · 2021Article
- Host Immune-Metabolic Adaptations Upon Mycobacterial Infections and Associated Co-Morbidities.Frontiers in immunology · 2021Review
- Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Chronic active non-lethal human-type tuberculosis in a high royal Bavarian officer of Napoleonic times-a mummy study.PloS one · 2021Article
- Human major infections: Tuberculosis, treponematoses, leprosy-A paleopathological perspective of their evolution.PloS one · 2021Article
- The paleopathological evidence on the origins of human tuberculosis: a review.Journal of preventive medicine and hygiene · 2020Review
- Circulation ofPathogens and global health · 2019Review
- Mitogenomic diversity in Sacred Ibis Mummies sheds light on early Egyptian practices.PloS one · 2019Article
8 more citing papers are in PubMed but not listed here.
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Bone and soft tissue samples from 85 ancient Egyptian mummies were analyzed for the presence of ancient Mycobacterium tuberculosis complex DNA (aDNA) and further characterized by spoligotyping. The specimens were obtained from individuals from different tomb complexes in Thebes West, Upper Egypt, which were used for upper social class burials between the Middle Kingdom (since ca. 2050 BC) and the Late Period (until ca. 500 BC). A total of 25 samples provided a specific positive signal for the amplification of a 123-bp fragment of the repetitive element IS6110, indicating the presence of M. tuberculosis DNA. Further PCR-based tests for the identification of subspecies failed due to lack of specific amplification products in the historic tissue samples. Of these 25 positive specimens, 12 could be successfully characterized by spoligotyping. The spoligotyping signatures were compared to those in an international database. They all show either an M. tuberculosis or an M. africanum pattern, but none revealed an M. bovis-specific pattern. The results from a Middle Kingdom tomb (used exclusively between ca. 2050 and 1650 BC) suggest that these samples bear an M. africanum-type specific spoligotyping signature. The samples from later periods provided patterns typical for M. tuberculosis. This study clearly demonstrates that spoligotyping can be applied to historic tissue samples. In addition, our results do not support the theory that M. tuberculosis originated from the M. bovis type but, rather, suggest that human M. tuberculosis may have originated from a precursor complex probably related to M. africanum.
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