Evidence map›Paper›PMID 12517863›Full record

ArticleJournal of clinical microbiology2003

Evaluation of a human group a rotavirus assay for on-site detection of bovine rotavirus.

Roger K Maes, Daniel L Grooms, Annabel G Wise, Cunqin Han, Valerie Ciesicki, Lora Hanson, Mary Lynne Vickers, Charles Kanitz, Robert Holland

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 61 citations in OpenAlex.

  1. Article
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  7. Molecular detection and genotyping of group A rotavirus in two wastewater treatment plants, Iran.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020
    Article
  8. Article
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  12. Diagnostics of dairy and beef cattle diarrhea.The Veterinary clinics of North America. Food animal practice · 2012
    Review
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  14. Article
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  16. Evaluation of relationship betweenComparative clinical pathology · 2010
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Roger K MaesDiagnostic Center for Population and Animal Health and Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824, USA. maes@ahdl.msu.edu
Daniel L Grooms
Annabel G Wise
Cunqin Han
Valerie Ciesicki
Lora Hanson
Mary Lynne Vickers
Charles Kanitz
Robert Holland
Département Santé Animale · FRIowa State University · USMichigan State University · USPurdue University West Lafayette · USUniversity of Kentucky · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neonatal diarrhea induced by bovine group A rotavirus causes significant economic loss in the dairy and beef industry due to increased morbidity and mortality, treatment costs, and reduced growth rates. The objective of this study was to evaluate a human group A rotavirus assay (ImmunoCardSTAT Rotavirus [ICS-RV]) as an on-site diagnostic test for bovine rotavirus. When used with a collection of bovine diarrhea samples submitted to the Virology Section of the Diagnostic Center for Population and Animal Health at Michigan State University and compared to a bovine group A rotavirus-specific reverse transcription-PCR (RT-PCR), the ICS-RV assay had a sensitivity and specificity of 87.0 and 93.6%, respectively. A commercially available group A rotavirus enzyme-linked immunosorbent assay (ELISA) (Pathfinder; Sanofi Diagnostics, Redmond, Wash.), when used with the same fecal sample collection and compared to the same RT-PCR, had a sensitivity and specificity of 78.3 and 67.7%, respectively. Subsequently, the ICS-RV assay, RT-PCR, and a different commercially available group A rotavirus ELISA (Rotaclone; Meridian Diagnostics, Cincinnati, Ohio) were used to evaluate fecal samples collected from neonatal calves experimentally infected with bovine rotavirus. When diarrheic fecal samples that were positive for bovine rotavirus by RT-PCR were evaluated, the ICS-RV assay and the Rotaclone assay detected bovine rotavirus 85 and 95% of the time, respectively. Based on these studies, the ICS-RV assay appears to be an excellent test for detecting group A bovine rotaviruses. This assay may be useful as an on-site diagnostic test for veterinarians as an aid in the management of bovine neonatal diarrhea.

Indexed as

Reagent Kits, DiagnosticAnimalsCattleCattle DiseasesHumansRotavirusRotavirus InfectionsSensitivity and SpecificityReagent Kits, Diagnostic

Identifiers

PMID12517863
PMCPMC149593
OpenAlexW2112749994

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.