ArticleJournal of clinical microbiology2003
Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed.
- Cross-kingdom microbial interactions in the gut during inflammatory bowel disease.Journal of translational medicine · 2026Review
- Infectious disease risk after the October 2024 flash flood in Valencia, Spain: Disaster evolution, strategic scenario analysis, and extrapolative baseline for a One Health assessment.One health (Amsterdam, Netherlands) · 2025Article
- The history of entamoebiasis.Parasitology · 2025Review
- Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples.Scientific reports · 2024Article
- Development of a salivary IgA detection method for accurate diagnosis of amebiasis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- The Epidemiology ofMedicina (Kaunas, Lithuania) · 2022Article
- A Comparative Analysis of Microscopy, Coproantigen Serology, and Nested Multiplex PCR in the Laboratory Diagnosis of Entamoeba histolytica Infection.Journal of laboratory physicians · 2022Article
- Genetic diversity ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Accurate laboratory diagnosis of human intestinal and extra-intestinal amoebiasis.Gastroenterology and hepatology from bed to bench · 2022Review
- Intestinal Parasite Infections and Associated Risk Factors among Pre-School Aged Children in Kibera Informal Settlement, Nairobi, Kenya.The East African health research journal · 2022Article
- Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal ParasitesMicroorganisms · 2021Article
- Parasitic Infections of the Gastrointestinal Track and Liver.Gastroenterology clinics of North America · 2021Review
- Intestinal protozoan infections shape fecal bacterial microbiota in children from Guinea-Bissau.PLoS neglected tropical diseases · 2021Article
- Undifferentiated Embryonal Sarcoma of the Liver Presents as a Molecular Mimic of Parasitic Infection.Cureus · 2020Article
- Review
- Update on laboratory diagnosis of amoebiasis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification.Acta tropica · 2018Article
- A Cross-Sectional Study on Intestinal Parasitic Infections in Children in Suburban Public Primary Schools, Saraburi, the Central Region of Thailand.The American journal of tropical medicine and hygiene · 2018Article
- Article
- Development and initial evaluation of a lateral flow dipstick test for antigen detection of Entamoeba histolytica in stool sample.Pathogens and global health · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differential diagnosis of Entamoeba histolytica (pathogenic) and Entamoeba dispar (nonpathogenic), which are two morphologically identical species of amebae, is essential both for treatment decision and public health knowledge. The study reported here was designed to choose a reference differentiation technique. Stool samples (n = 95) were tested by microscopy, TechLab enzyme-linked immunosorbent assays (ELISAs), and an in-house PCR. The target for the PCR amplification was a small region (135 bp) of the SSU rRNA selected to increase the sensitivity of the test. Sixty-eight specimens tested positive by PCR: 2 for E. histolytica and 66 for E. dispar. For detection of E. dispar, ELISA performance was lower than that of microscopy in this reference context, while PCR was much more sensitive than microscopy. Given the low proportion of E. histolytica cases, test performance for this species is difficult to assess. However, for differentiation, PCR performed well on simulated samples, while ELISA gave a discordant result for one of the two samples PCR positive for E. histolytica during the study. This report also confirms that E. dispar infection is significantly higher among travelers and underlines the possibility of acquiring E. histolytica infection in regions that are not areas of endemicity. Because of its lower sensitivity, the interest of ELISA for Entamoeba detection and differentiation in stools seems questionable in nontropical regions. On the other hand, results suggest that PCR should be useful as a reference test for sensitive differentiation of both species and to contribute to physicians' decision in treatment of E. histolytica- or E. dispar-infected patients.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.