ArticleJournal of clinical microbiology2003
Detection, isolation, and molecular subtyping of Escherichia coli O157:H7 and Campylobacter jejuni associated with a large waterborne outbreak.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Ultrasonographic Demonstration of Severe Right-Sided Colitis in Concurrent EnterohemorrhagicDiagnostics (Basel, Switzerland) · 2026Article
- Recovery of the gut microbiome following enteric infection and persistence of antimicrobial resistance genes in specific microbial hosts.Scientific reports · 2023Article
- Quantitative microbial risk assessment for occupational health of temporary entrants and staffs equipped with various grade PPE and exposed to microbial bioaerosols in two WWTPs.International archives of occupational and environmental health · 2021Article
- Rapid detection ofFood science and biotechnology · 2020Article
- Case-Control Assessment of the Roles of Noroviruses, Human Bocaviruses 2, 3, and 4, and Novel Polyomaviruses and Astroviruses in Acute Childhood Diarrhea.Journal of the Pediatric Infectious Diseases Society · 2017Article
- Shiga Toxin (Verotoxin)-producingFood safety (Tokyo, Japan) · 2017Review
- Occurrence of multidrug resistantVeterinary world · 2017Article
- Isolation of atypical enteropathogenic and shiga toxin encoding Escherichia coli strains from poultry in Tehran, Iran.Gastroenterology and hepatology from bed to bench · 2016Article
- Escherichia coli O157:H7 bacteriophage Φ241 isolated from an industrial cucumber fermentation at high acidity and salinity.Frontiers in microbiology · 2015Article
- Article
- Transport ofWater resources research · 2013Article
- Comparison of three different methods for detection of Shiga toxin-producing Escherichia coli in a tertiary pediatric care center.Journal of clinical microbiology · 2013Article
- Escherichia coli O157:H7-Clinical aspects and novel treatment approaches.Frontiers in cellular and infection microbiology · 2012Review
- Review
- Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water.International journal of microbiology · 2011Article
- Recovery and detection of Escherichia coli O157:H7 in surface water, using ultrafiltration and real-time PCR.Applied and environmental microbiology · 2009Article
- Campylobacter immunity and coinfection following a large outbreak in a farming community.Journal of clinical microbiology · 2009Article
- Rapid determination of Escherichia coli O157:H7 lineage types and molecular subtypes by using comparative genomic fingerprinting.Applied and environmental microbiology · 2008Article
- Isolation of Salmonella enterica and Shiga-toxigenic Escherichia coli O157 from feces of animals in public contact areas of United States zoological parks.Applied and environmental microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The largest reported outbreak of waterborne Escherichia coli O157:H7 in the United States occurred in upstate New York following a county fair in August 1999. Culture methods were used to isolate E. coli O157:H7 from specimens from 128 of 775 patients with suspected infections. Campylobacter jejuni was also isolated from stools of 44 persons who developed diarrheal illness after attending this fair. There was one case of a confirmed coinfection with E. coli O157:H7 and C. jejuni. Molecular detection of stx(1) and stx(2) Shiga toxin genes, immunomagnetic separation (IMS), and selective culture enrichment were utilized to detect and isolate E. coli O157:H7 from an unchlorinated well and its distribution points, a dry well, and a nearby septic tank. PCR for stx(1) and stx(2) was shown to provide a useful screen for toxin-producing E. coli O157:H7, and IMS subculture improved recovery. Pulsed-field gel electrophoresis (PFGE) was used to compare patient and environmental E. coli O157:H7 isolates. Among patient isolates, 117 of 128 (91.5%) were type 1 or 1a (three or fewer bands different). Among the water distribution system isolates, 13 of 19 (68%) were type 1 or 1a. Additionally, PFGE of C. jejuni isolates revealed that 29 of 35 (83%) had indistinguishable PFGE patterns. The PFGE results implicated the water distribution system as the main source of the E. coli O157:H7 outbreak. This investigation demonstrates the potential for outbreaks involving more than one pathogen and the importance of analyzing isolates from multiple patients and environmental samples to develop a better understanding of bacterial transmission during an outbreak.
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