ArticleJournal of clinical microbiology2003
Molecular assays for detection of human metapneumovirus.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed, 180 citations in OpenAlex.
- A scoring system for management of acute pharyngo-tonsillitis in adults.Auris, nasus, larynx · 2009Trial
- Review
- A Review of Respiratory Infection Caused by the Human Metapneumovirus: Occurrence, Epidemiology, Pathogenesis, Public Health Outcomes, and Intervention Strategies.Health science reports · 2026Article
- Article
- Advances in Human Metapneumovirus Research: Clinical Impact, Diagnostic Innovations, and Therapeutic Challenges.Pathogens (Basel, Switzerland) · 2025Review
- Preventing the Silent Threat: A Perspective on Preparing Bangladesh for Human Metapneumovirus (HMPV).Health science reports · 2025Article
- Molecular detection and genotyping of HMPV in patients with severe acute respiratory infection in India.Annals of medicine · 2024Article
- Suitcase Lab for Rapid Detection of SARS-CoV-2 Based on Recombinase Polymerase Amplification Assay.Analytical chemistry · 2021Article
- The COVID 19 novel coronavirus pandemic 2020: seaweeds to the rescue? Why does substantial, supporting research about the antiviral properties of seaweed polysaccharides seem to go unrecognized by the pharmaceutical community in these desperate times?Journal of applied phycology · 2020Article
- Metagenomic analysis using next-generation sequencing of pathogens in bronchoalveolar lavage fluid from pediatric patients with respiratory failure.Scientific reports · 2019Article
- Review
- The role of human Metapneumovirus genetic diversity and nasopharyngeal viral load on symptom severity in adults.Virology journal · 2018Article
- Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses.Journal of virological methods · 2018Article
- Immune Response to Human Metapneumovirus Infection: What We Have Learned from the Mouse Model.Pathogens (Basel, Switzerland) · 2015Review
- Outbreak of coinfection with human metapneumovirus and measles virus resulting in the death of a child at a hospital in China.American journal of infection control · 2015Article
- Development of real-time RT-PCR for detection of human metapneumovirus and genetic analysis of circulating strains (2009-2011) in Pune, India.Archives of virology · 2014Article
- Survey of causative agents for acute respiratory infections among patients in Khartoum-State, Sudan, 2010-2011.Virology journal · 2013Article
- Article
- Real-time reverse transcriptase PCR assay for improved detection of human metapneumovirus.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
- The viral etiology of an influenza-like illness during the 2009 pandemic.Journal of medical virology · 2012Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent description of the respiratory pathogen human metapneumovirus (hMPV) has highlighted a deficiency in current diagnostic techniques for viral agents associated with acute lower respiratory tract infections. We describe two novel approaches to the detection of viral RNA by use of reverse transcriptase PCR (RT-PCR). The PCR products were identified after capture onto a solid-phase medium by hybridization with a sequence-specific, biotinylated oligonucleotide probe. The assay was applied to the screening of 329 nasopharyngeal aspirates sampled from patients suffering from respiratory tract disease. These samples were negative for other common microbial causes of respiratory tract disease. We were able to detect hMPV sequences in 32 (9.7%) samples collected from Australian patients during 2001. To further reduce result turnaround times we designed a fluorogenic TaqMan oligoprobe and combined it with the existing primers for use on the LightCycler platform. The real-time RT-PCR proved to be highly reproducible and detected hMPV in an additional 6 out of 62 samples (9.6%) tested during the comparison of the two diagnostic approaches. We found the real-time RT-PCR to be the test of choice for future investigation of samples for hMPV due to its speed, reproducibility, specificity, and sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.