ArticleJournal of clinical microbiology2003
Evaluation of a PCR assay for detection of Streptococcus pneumoniae in respiratory and nonrespiratory samples from adults with community-acquired pneumonia.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Usefulness of sputum gram stain for etiologic diagnosis in community-acquired pneumonia: a systematic review and meta-analysis.BMC infectious diseases · 2019Pooled it
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- Analysis of Potential Genes, Acute Phase Proteins and Hormonal Profiles Associated with Methicillin-ResistantVeterinary sciences · 2025Article
- Molecular Detection of Antibiotic Resistance Genes Using Respiratory Sample from Pneumonia Patients.Antibiotics (Basel, Switzerland) · 2025Article
- Single nucleotide polymorphisms, gene expression and evaluation of immunological, antioxidant, and pathological parameters associated with bacterial pneumonia in Barki sheep.Irish veterinary journal · 2025Article
- Development and evaluation of a real-time multienzyme isothermal rapid amplification assay for rapid detection of Streptococcus pneumoniae.Scientific reports · 2024Article
- Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology.The Journal of molecular diagnostics : JMD · 2023Review
- Value of pneumococcal PCR in respiratory samples for exclusion of pneumococcal pneumonia.JAC-antimicrobial resistance · 2023Article
- BK virus associated with small cell carcinoma of bladder in a patient with renal transplant.BMJ case reports · 2022Article
- Uses of pathogen detection data to estimate vaccine direct effects in case-control studies.Journal of the Royal Society, Interface · 2020Article
- Impact of Laboratory Methods and Gene Targets on Detection ofReports of biochemistry & molecular biology · 2020Article
- BAL fluid analysis in the identification of infectious agents in patients with hematological malignancies and pulmonary infiltrates.Folia microbiologica · 2020Article
- How recent advances in molecular tests could impact the diagnosis of pneumonia.Expert review of molecular diagnostics · 2016Review
- The upper respiratory tract microbiome of hospitalised patients with community-acquired pneumonia of unknown aetiology: a pilot study.Pneumonia (Nathan Qld.) · 2015Article
- Genomic load from sputum samples and nasopharyngeal swabs for diagnosis of pneumococcal pneumonia in HIV-infected adults.Journal of clinical microbiology · 2014Article
- Phylogeography of Rickettsia rickettsii genotypes associated with fatal Rocky Mountain spotted fever.The American journal of tropical medicine and hygiene · 2014Article
- Evaluation of yield of currently available diagnostics by sample type to optimize detection of respiratory pathogens in patients with a community-acquired pneumonia.Influenza and other respiratory viruses · 2014Observational
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus pneumoniae is the most common cause of community-acquired pneumonia, but it is undoubtedly underdiagnosed. We used a nested PCR assay (targeting the pneumolysin gene) to detect S. pneumoniae DNA in multiple sample types from 474 adults with community-acquired pneumonia and 183 control patients who did not have pneumonia. Plasma or buffy coat samples were PCR positive in only 6 of the 21 patients with positive blood cultures for S. pneumoniae and in 12 other patients (4 of whom had no other laboratory evidence of S. pneumoniae infection). Buffy coat samples from two control patients (neither having evidence of S. pneumoniae infection), but no control plasma samples, were PCR positive. Although pneumococcal antigen was detected in the urine from 120 of 420 (29%) patients, only 4 of 227 (2%) urine samples tested were PCR positive. Overall, 256 of 318 (81%) patients had PCR-positive sputum samples, including 58 of 59 samples from which S. pneumoniae was cultured. Throat swab samples from 229 of 417 (55%) patients were PCR positive and, in those who produced sputum, 96% also had positive PCR results from sputum. Throat swabs from 73 of 126 (58%) control patients were also PCR positive. We conclude that the pneumolysin PCR assay adds little to existing diagnostic tests for S. pneumoniae and is unable to distinguish colonization from infection when respiratory samples are tested.
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