Evidence map›Paper›PMID 12516858›Full record

ReviewPlant molecular biology2002

Analysing protein-protein interactions with the yeast two-hybrid system.

Barry Causier, Brendan Davies

Abstract readReview
PubMed Publisher
In one paragraph

Review in Plant molecular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Protein-Protein Interactions: Yeast Two Hybrid.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. The Dynamics of Flower Development inPlants (Basel, Switzerland) · 2021
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Barry CausierSchool of Biology, University of Leeds, Leeds LS2 9J7, UK. b.e.causier@leeds.ac.uk
Brendan Davies

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant research is moving into the post-genomic era. Proteomic-based strategies are now being developed to study functional aspects of the genes predicted from the various genome-sequencing initiatives. All biological processes depend on interactions formed between proteins and the mapping of such interactions on a global scale is providing interesting functional insights. One of the techniques that has proved itself invaluable in the mapping of protein-protein interactions is the yeast two-hybrid system. This system is a sensitive molecular genetic approach for studying protein-protein interactions in vivo. In this review we will introduce the yeast two-hybrid system, discuss modifications of the system that may be of interest to the plant science community and suggest potential applications of the technology.

Indexed as

Two-Hybrid System TechniquesGenes, ReporterGenetic VectorsPlant ProteinsProtein BindingRecombinant Fusion ProteinsSaccharomyces cerevisiaePlant ProteinsRecombinant Fusion Proteins

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.