ArticleThe Journal of experimental medicine2003
Natural killer cell inhibitory receptors block actin cytoskeleton-dependent recruitment of 2B4 (CD244) to lipid rafts.
Article in The Journal of experimental medicine, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 122 citations in OpenAlex.
- Article
- Exhausted NK cells and cytokine storms in COVID-19: Whether NK cell therapy could be a therapeutic choice.Human immunology · 2022Review
- The Role of the Cytoskeleton in Regulating the Natural Killer Cell Immune Response in Health and Disease: From Signaling Dynamics to Function.Frontiers in cell and developmental biology · 2021Review
- Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity.Frontiers in immunology · 2020Review
- Reconstituting NK Cells After Allogeneic Stem Cell Transplantation Show Impaired Response to the Fungal PathogenFrontiers in immunology · 2020Article
- NK cell recognition of hematopoietic cells by SLAM-SAP families.Cellular & molecular immunology · 2019Review
- Subversion of natural killer cell responses by a cytomegalovirus-encoded soluble CD48 decoy receptor.PLoS pathogens · 2019Article
- Impedance-based analysis of Natural Killer cell stimulation.Scientific reports · 2018Article
- Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1.The EMBO journal · 2018Article
- Natural Killer Cell Education and the Response to Infection and Cancer Therapy: Stay Tuned.Trends in immunology · 2018Review
- Altered expression of miR-181a and miR-146a does not change the expression of surface NCRs in human NK cells.Scientific reports · 2017Article
- Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes.Frontiers in immunology · 2017Article
- Redirected Primary Human Chimeric Antigen Receptor Natural Killer Cells As an "Off-the-Shelf Immunotherapy" for Improvement in Cancer Treatment.Frontiers in immunology · 2017Review
- Modulation of natural killer cell functions by interactions between 2B4 and CD48 in cis and in trans.Open biology · 2016Article
- Zinc-Induced Polymerization of Killer-Cell Ig-like Receptor into Filaments Promotes Its Inhibitory Function at Cytotoxic Immunological Synapses.Molecular cell · 2016Article
- Responses to Microbial Challenges by SLAMF Receptors.Frontiers in immunology · 2016Review
- Natural killer (NK) cell profiles in blood and tumour in women with large and locally advanced breast cancer (LLABC) and their contribution to a pathological complete response (PCR) in the tumour following neoadjuvant chemotherapy (NAC): differential restoration of blood profiles by NAC and surgery.Journal of translational medicine · 2015Article
- L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.Journal of leukocyte biology · 2014Article
- Cytomegalovirus m154 hinders CD48 cell-surface expression and promotes viral escape from host natural killer cell control.PLoS pathogens · 2014Article
- Natural killer cell regulation - beyond the receptors.F1000prime reports · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
A dynamic balance of positive and negative signals regulates target cell lysis by natural killer (NK) cells upon engagement of a variety of different activation receptors and of inhibitory receptors that recruit the tyrosine phosphatase SHP-1. However, the step at which activation signals are blocked by SHP-1 is not known. We have been using activation receptor 2B4 (CD244) to study the influence of inhibitory receptors on NK cell activation. Engagement of inhibitory receptors by HLA class I on target cells blocks phosphorylation of 2B4, placing the inhibitory step at the level, or upstream of 2B4 phosphorylation. Here we show that phosphorylated 2B4, after engagement with either antibodies or target cells that express the 2B4 ligand, is found exclusively in a detergent-resistant membrane fraction that contains lipid rafts. Integrity of lipid rafts was essential for phosphorylation and activating function of 2B4. Coengagement of inhibitory receptors blocked 2B4 phosphorylation and 2B4 association with detergent-resistant membranes, indicating that inhibitory receptors function upstream of raft-dependent signals. Recruitment of 2B4 into detergent-resistant membrane fractions and 2B4 phosphorylation were dependent on actin polymerization. Blocking actin cytoskeleton-dependent raft recruitment of different receptors may be a general mechanism by which inhibitory receptors control NK cell activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.