ArticleRNA (New York, N.Y.)2002
An H/ACA guide RNA directs U2 pseudouridylation at two different sites in the branchpoint recognition region in Xenopus oocytes.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 49 citations in OpenAlex.
- Targeted pseudouridylation: An approach for suppressing nonsense mutations in disease genes.Molecular cell · 2023Article
- "Lost and Found": snoRNA Annotation in the Xenopus Genome and Implications for Evolutionary Studies.Molecular biology and evolution · 2020Article
- Small, Smaller, Smallest: Minimal Structural Requirements for a Fully Functional Box C/D Modification Guide RNA.Biomolecules · 2019Article
- Variants in PUS7 Cause Intellectual Disability with Speech Delay, Microcephaly, Short Stature, and Aggressive Behavior.American journal of human genetics · 2018Article
- Orchestrated positioning of post-transcriptional modifications at the branch point recognition region of U2 snRNA.RNA (New York, N.Y.) · 2018Article
- Dual nature of pseudouridylation in U2 snRNA: Pus1p-dependent and Pus1p-independent activities in yeasts and higher eukaryotes.RNA (New York, N.Y.) · 2017Article
- RNA modification in Cajal bodies.RNA biology · 2017Review
- The TOR signaling pathway regulates starvation-induced pseudouridylation of yeast U2 snRNA.RNA (New York, N.Y.) · 2016Article
- Detection and quantification of RNA 2'-O-methylation and pseudouridylation.Methods (San Diego, Calif.) · 2016Article
- Transcriptome-wide dynamics of RNA pseudouridylation.Nature reviews. Molecular cell biology · 2015Review
- Revisiting the structure/function relationships of H/ACA(-like) RNAs: a unified model for Euryarchaea and Crenarchaea.Nucleic acids research · 2015Article
- Insight into the mechanisms and functions of spliceosomal snRNA pseudouridylation.World journal of biological chemistry · 2014Review
- RNA-guided isomerization of uridine to pseudouridine--pseudouridylation.RNA biology · 2014Review
- Novel small Cajal-body-specific RNAs identified in Drosophila: probing guide RNA function.RNA (New York, N.Y.) · 2013Article
- RNA pseudouridylation: new insights into an old modification.Trends in biochemical sciences · 2013Review
- Synthesis and labeling of RNA in vitro.Current protocols in molecular biology · 2013Article
- Pearls are novel Cajal body-like structures in the Xenopus germinal vesicle that are dependent on RNA pol III transcription.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Pseudouridine synthase 1: a site-specific synthase without strict sequence recognition requirements.Nucleic acids research · 2012Article
- Pseudouridines in spliceosomal snRNAs.Protein & cell · 2011Review
- Targeted 2'-O methylation at a nucleotide within the pseudoknot of telomerase RNA reduces telomerase activity in vivo.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
U2 is the most extensively modified of all spliceosomal snRNAs. We previously showed that at least some of the internally modified nucleotides in U2 snRNA are required for snRNP biogenesis and pre-mRNA splicing. Recent work from several laboratories suggests that nuclear guide RNAs facilitate U2 snRNA internal modification, including pseudouridylation and 2'-O-methylation. Here, we present a novel approach to identifying guide RNAs for U2 pseudouridylation. Several Xenopus oocyte nuclear RNAs were affinity selected with U2 snRNA substituted with 5-fluorouridine, a pseudouridylation inhibitor that sequesters pseudouridylases. One of these RNAs was sequenced and found to be a novel RNA of 134 nt. This small RNA contains an H/ACA motif and folds into a typical H/ACA RNA structure, and its authenticity as an H/ACA RNA was confirmed by immunoprecipitation analysis. The RNA contains two guide sequences for pseudouridylation (psi) of U2 snRNA at positions 34 and 44 in the branch-site recognition region, and we demonstrate that this RNA indeed guides the formation of psi34 and psi44 in U2 using a Xenopus oocyte reconstitution system. Therefore, this novel RNA was designated pugU2-34/44, for pseudouridylation guide for U2 snRNA U34 and U44. Intranuclear localization analyses indicate that pugU2-34/44 resides within the nucleoplasm rather than nucleoli or Cajal bodies where other guide RNAs have been localized. Our results clarify the mechanism of U2 snRNA pseudouridylation in Xenopus oocytes, and have interesting implications with regard to the intranuclear localization of U2 snRNA pseudouridylation.
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Identifiers
12515384PMC1370357W1877746820What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.