ArticleThe EMBO journal2003
The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 166 citations in OpenAlex.
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- Genomic and Functional Variation of the ChlorophyllMicroorganisms · 2022Article
- Photoglobin, a distinct family of non-heme binding globins, defines a potential photosensor in prokaryotic signal transduction systems.Computational and structural biotechnology journal · 2022Article
- Functional Annotation from Structural Homology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Biosynthesis of the Tricyclic Aromatic Type II Polyketide Rishirilide: New Potential Third Ring Oxygenation after Three Cyclization Steps.Molecular biotechnology · 2021Article
- Exploring the Diversity of Fungal DyPs in Mangrove Soils to Produce and Characterize Novel Biocatalysts.Journal of fungi (Basel, Switzerland) · 2021Article
- A Dynamic Substrate is Required for MhuD-Catalyzed Degradation of Heme to Mycobilin.Biochemistry · 2021Article
- Biosynthesis of aromatic polyketides in microorganisms using type II polyketide synthases.Microbial cell factories · 2020Review
- Early Steps in the Biosynthetic Pathway of Rishirilide B.Molecules (Basel, Switzerland) · 2020Article
- Genotyping-Guided Discovery of Persiamycin A From Sponge-Associated HalophilicFrontiers in microbiology · 2020Article
- A role for antibiotic biosynthesis monooxygenase domain proteins in fidelity control during aromatic polyketide biosynthesis.Nature communications · 2019Article
- How a cofactor-free protein environment lowers the barrier to OThe Journal of biological chemistry · 2019Article
- The functional differentiation of the post-PKS tailoring oxygenases contributed to the chemical diversities of atypical angucyclines.Synthetic and systems biotechnology · 2018Review
- In silico characterization of a novel putative aerotaxis chemosensory system in the myxobacterium, Corallococcus coralloides.BMC genomics · 2018Article
- Pseudomonas aeruginosa PumA acts on an endogenous phenazine to promote self-resistance.Microbiology (Reading, England) · 2018Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ActVA-Orf6 monooxygenase from Streptomyces coelicolor that catalyses the oxidation of an aromatic intermediate of the actinorhodin biosynthetic pathway is a member of a class of small monooxygenases that carry out oxygenation without the assistance of any of the prosthetic groups, metal ions or cofactors normally associated with activation of molecular oxygen. The overall structure is a ferredoxin-like fold with a novel dimeric assembly, indicating that the widely represented ferredoxin fold may sustain yet another functionality. The resolution (1.3 A) of the enzyme structure and its complex with substrate and product analogues allows us to visualize the mechanism of binding and activation of the substrate for attack by molecular oxygen, and utilization of two gates for the reaction components including a proton gate and an O(2)/H(2)O gate with a putative protein channel. This is the first crystal structure of an enzyme involved in the tailoring of a type II aromatic polyketide and illustrates some of the enzyme-substrate recognition features that may apply to a range of other enzymes involved in modifying a polyketide core structure.
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