ArticleApplied and environmental microbiology2003
A food-grade approach for functional analysis and modification of native plasmids in Lactococcus lactis.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Genetically Modified Lactic Acid Bacteria in the EU Food Chain: Applications, Benefits, and Risk Assessment.International journal of molecular sciences · 2026Review
- Synergistic and off-target effects of bacteriocins in a simplified human intestinal microbiome: implications forGut microbes · 2025Article
- Advanced Strategies for Food-Grade Protein Production: A NewMicroorganisms · 2019Article
- Vector Development Timeline for Mucosal Vaccination and Treatment of Disease UsingFrontiers in microbiology · 2018Review
- A review on Lactococcus lactis: from food to factory.Microbial cell factories · 2017Review
- Metabolic and transcriptional analysis of acid stress in Lactococcus lactis, with a focus on the kinetics of lactic acid pools.PloS one · 2013Article
- Insights into Lantibiotic Immunity Provided by Bioengineering of LtnI.Antimicrobial agents and chemotherapy · 2012Article
- Homologues and bioengineered derivatives of LtnJ vary in ability to form D-alanine in the lantibiotic lacticin 3147.Journal of bacteriology · 2012Article
- Studies with bioengineered Nisin peptides highlight the broad-spectrum potency of Nisin V.Microbial biotechnology · 2010Article
- Manipulation of charged residues within the two-peptide lantibiotic lacticin 3147.Microbial biotechnology · 2010Article
- Production of the Bsa lantibiotic by community-acquired Staphylococcus aureus strains.Journal of bacteriology · 2010Article
- A kinetic study on the plasmid stability of three Lactococcus lactis strains.Journal of industrial microbiology & biotechnology · 2007Article
- Insertional mutagenesis to generate lantibiotic resistance in Lactococcus lactis.Applied and environmental microbiology · 2007Article
- Relatedness between the two-component lantibiotics lacticin 3147 and staphylococcin C55 based on structure, genetics and biological activity.BMC microbiology · 2007Article
- Sequence analysis of the lactococcal plasmid pNP40: a mobile replicon for coping with environmental hazards.Journal of bacteriology · 2006Article
- Posttranslational conversion of L-serines to D-alanines is vital for optimal production and activity of the lantibiotic lacticin 3147.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Bioengineering: a bacteriocin perspective.BioengineeredArticle
- Efficacy of a Lactococcus lactis ΔpyrG vaccine delivery platform expressing chromosomally integrated hly from Listeria monocytogenes.Bioengineered bugsArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While plasmids from lactic acid bacteria possess many traits that are of industrial value, their exploitation is often frustrated by an inability to conduct food-grade engineering of native plasmids or to readily screen for their transfer. Here we describe a system that uses a RepA(+) temperature-sensitive helper plasmid and a RepA(-) cloning vector to overcome these problems while maintaining the food-grade status of the native plasmid. This strategy was used to precisely delete ltnA1 alone, or in conjunction with ltnA2 (encoding the structural proteins of the lantibiotic lacticin 3147), from the native 60.2-kb plasmid pMRC01 and to select for the transfer of pMRC01 between Lactococcus lactis strains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.