ArticleApplied and environmental microbiology2003
Bacteria associated with cysts of the soybean cyst nematode (Heterodera glycines).
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 87 citations in OpenAlex.
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- The Composition and Function of Bacterial Communities Associated with the Northern Root-Knot Nematode (Microorganisms · 2025Article
- Crop Rotation with Resistant Potato andJournal of nematology · 2025Article
- Microbiome dynamics associated with the infection of grey field slugs by the biocontrol nematodeFrontiers in microbiology · 2025Article
- Microbiome of Xiphinema elongatum (Nematoda, Longidoridae), isolated from water berry.Scientific reports · 2024Article
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- Toxic Effects of the Trap CropJournal of nematology · 2024Article
- Evaluation of Solanaceous Species as Nonhost Trap Crops forJournal of nematology · 2023Article
- Soybean microbiome composition and the impact of host plant resistance.Frontiers in plant science · 2023Article
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- Belowground Chemical Interactions: An Insight Into Host-Specific Behavior ofFrontiers in plant science · 2021Article
- Impact of Wheat on Soybean Cyst Nematode Population Density in Double-Cropping Soybean Production.Frontiers in plant science · 2021Article
- Bacterial Microbiota Isolated from Cysts ofPlants (Basel, Switzerland) · 2020Article
- Transcriptome profiling ofAccess microbiology · 2020Article
- Plants and Associated Soil Microbiota Cooperatively Suppress Plant-Parasitic Nematodes.Frontiers in microbiology · 2020Review
- Bacteria isolated from the cuticle of plant-parasitic nematodes attached to and antagonized the root-knot nematode Meloidogyne hapla.Scientific reports · 2019Article
- Rhizosphere Microbiomes from Root Knot Nematode Non-infested Plants Suppress Nematode Infection.Microbial ecology · 2019Article
- Biocontrol of Cereal Crop Diseases Using Streptomycetes.Pathogens (Basel, Switzerland) · 2019Review
- Seasonal Variation and Crop Sequences Shape the Structure of Bacterial Communities in Cysts of Soybean Cyst Nematode.Frontiers in microbiology · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The soybean cyst nematode (SCN), Heterodera glycines, causes economically significant damage to soybeans (Glycine max) in many parts of the world. The cysts of this nematode can remain quiescent in soils for many years as a reservoir of infection for future crops. To investigate bacterial communities associated with SCN cysts, cysts were obtained from eight SCN-infested farms in southern Ontario, Canada, and analyzed by culture-dependent and -independent means. Confocal laser scanning microscopy observations of cyst contents revealed a microbial flora located on the cyst exterior, within a polymer plug region and within the cyst. Microscopic counts using 5-(4,6-dichlorotriazine-2-yl)aminofluorescein staining and in situ hybridization (EUB 338) indicated that the cysts contained (2.6 +/- 0.5) x 10(5) bacteria (mean +/- standard deviation) with various cellular morphologies. Filamentous fungi were also observed. Live-dead staining indicated that the majority of cyst bacteria were viable. The probe Nile red also bound to the interior polymer, indicating that it is lipid rich in nature. Bacterial community profiles determined by denaturing gradient gel electrophoresis analysis were simple in composition. Bands shared by all eight samples included the actinobacterium genera Actinomadura and STREPTOMYCES: A collection of 290 bacteria were obtained by plating macerated surface-sterilized cysts onto nutrient broth yeast extract agar or on actinomycete medium. These were clustered into groups of siblings by repetitive extragenic palindromic PCR fingerprinting, and representative isolates were tentatively identified on the basis of 16S rRNA gene sequence. Thirty phylotypes were detected, with the collection dominated by Lysobacter and Variovorax spp. This study has revealed the cysts of this important plant pathogen to be rich in a variety of bacteria, some of which could presumably play a role in the ecology of SCN or have potential as biocontrol agents.
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