ArticleApplied and environmental microbiology2003
Enhanced heterologous expression of two Streptomyces griseolus cytochrome P450s and Streptomyces coelicolor ferredoxin reductase as potentially efficient hydroxylation catalysts.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 65 citations in OpenAlex.
- Structural Insights into the Interaction of Terpenoids withBiomolecules & therapeutics · 2024Article
- Whole-Genome Sequencing of a Chlorimuron-Ethyl-Degrading Strain: Chenggangzhangella methanolivorans CHL1 and Its Degrading Enzymes.Microbiology spectrum · 2022Article
- Article
- A bifunctional enzyme belonging to cytochrome P450 family involved in the O-dealkylation and N-dealkoxymethylation toward chloroacetanilide herbicides in Rhodococcus sp. B2.Microbial cell factories · 2021Article
- Streptomycetes as platform for biotechnological production processes of drugs.Applied microbiology and biotechnology · 2021Review
- Identification and catalytic properties of new epoxide hydrolases from the genomic data of soil bacteria.Enzyme and microbial technology · 2020Article
- Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 fromSynthetic and systems biotechnology · 2020Article
- Functional Analysis of P450 Monooxygenase SrrO in the Biosynthesis of Butenolide-Type Signaling Molecules inBiomolecules · 2020Article
- Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.Natural product reports · 2017Review
- Chemical complexity of protein determines optimalJournal, genetic engineering & biotechnology · 2017Article
- Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.Microbial cell factories · 2016Article
- Identification of a cyclosporine-specific P450 hydroxylase gene through targeted cytochrome P450 complement (CYPome) disruption in Sebekia benihana.Applied and environmental microbiology · 2013Article
- In-silico and In-vitro based studies of Streptomyces peucetius CYP107N3 for oleic acid epoxidation.BMB reports · 2012Article
- A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling.BMC plant biology · 2011Article
- The plmS2-encoded cytochrome P450 monooxygenase mediates hydroxylation of phoslactomycin B in Streptomyces sp. strain HK803.Journal of bacteriology · 2005Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The herbicide-inducible, soluble cytochrome P450s CYP105A1 and CYP105B1 and their adjacent ferredoxins, Fd1 and Fd2, of Streptomyces griseolus were expressed in Escherichia coli to high levels. Conditions for high-level expression of active enzyme able to catalyze hydroxylation have been developed. Analysis of the expression levels of the P450 proteins in several different E. coli expression hosts identified E. coli BL21 Star(DE3)pLysS as the optimal host cell to express CYP105B1 as judged by CO difference spectra. Examination of the codons used in the CYP1051A1 sequence indicated that it contains a number of codons corresponding to rare E. coli tRNA species. The level of its expression was improved in the modified forms of E. coli BL21(DE3), which contain extra copies of rare codon E. coli tRNA genes. The activity of correctly folded cytochrome P450s was further enhanced by cloning a ferredoxin reductase from Streptomyces coelicolor downstream of CYP105A1 and CYP105B1 and their adjacent ferredoxins. Expression of CYP105A1 and CYP105B1 was also achieved in Streptomyces lividans 1326 by cloning the P450 genes and their ferredoxins into the expression vector pBW160. S. lividans 1326 cells containing CYP105A1 or CYP105B1 were able efficiently to dealkylate 7-ethoxycoumarin.
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