ArticleApplied and environmental microbiology2003
Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-Like protease from Streptomyces albogriseolus.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 136 citations in OpenAlex.
- Rewiring Amino Acid Flux for Efficient Transglutaminase Production inJournal of agricultural and food chemistry · 2026Article
- Microbial transglutaminase in food biotechnology: from biochemical mechanisms to industrial applications.Applied microbiology and biotechnology · 2026Review
- Rapid Identification of Superior Endogenous Signal Peptides for Heterologous Protein Secretion by Corynebacterium glutamicum Through Modular Cloning and Automation.Microbial biotechnology · 2026Article
- Introduction of multiple disulfide bonds increases the thermostability of transglutaminase.Scientific reports · 2025Article
- Random mutagenesis and disulfide bond formation improved thermostability in microbial transglutaminase.Applied microbiology and biotechnology · 2024Article
- Identification of multiple regulatory genes involved in TGase production inEngineering microbiology · 2023Article
- Transglutaminase in Foods and Biotechnology.International journal of molecular sciences · 2023Review
- Efficient secretory production of proline/alanine/serine (PAS) biopolymers in Corynebacterium glutamicum yielding a monodisperse biological alternative to polyethylene glycol (PEG).Microbial cell factories · 2022Article
- Rational design of a disulfide bridge increases the thermostability of microbial transglutaminase.Applied microbiology and biotechnology · 2022Article
- Single-chain tandem macrocyclic peptides as a scaffold for growth factor and cytokine mimetics.Communications biology · 2022Article
- Induction of glutamic acid production by copper in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2021Article
- Optimizing Protein-Glutaminase Expression in Bacillus subtilis.Current microbiology · 2021Article
- PASylated Thymosin α1: A Long-Acting Immunostimulatory Peptide for Applications in Oncology and Virology.International journal of molecular sciences · 2020Article
- Heterologous Expression of Recombinant Transglutaminase inJournal of microbiology and biotechnology · 2020Article
- Transglutaminases: part I-origins, sources, and biotechnological characteristics.World journal of microbiology & biotechnology · 2020Review
- Recombinant Protein Expression System inFrontiers in microbiology · 2018Review
- Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors.PloS one · 2017Article
- Improving the active expression of transglutaminase in Streptomyces lividans by promoter engineering and codon optimization.BMC biotechnology · 2016Article
- Corynebacterium glutamicum possesses β-N-acetylglucosaminidase.BMC microbiology · 2016Article
- Transcriptome and Metabolome Analyses in Exogenous FABP4- and FABP5-Treated Adipose-Derived Stem Cells.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transglutaminase secreted by Streptoverticillium mobaraense is a useful enzyme in the food industry. A fragment of transglutaminase was secreted by Corynebacterium glutamicum when it was coupled on a plasmid to the promoter and signal peptide of a cell surface protein from C. glutamicum. We analyzed the signal peptide and the pro-domain of the transglutaminase gene and found that the signal peptide consists of 31 amino acid residues and the pro-domain consists of 45 residues. When the pro-domain of the transglutaminase was used, the pro-transglutaminase was secreted efficiently by C. glutamicum but had no enzymatic activity. However, when the plasmid carrying the S. mobaraense transglutaminase also encoded SAM-P45, a subtilisin-like serine protease derived from Streptomyces albogriseolus, the peptide bond to the C side of 41-Ser of the pro-transglutaminase was hydrolyzed, and the pro-transglutaminase was converted to an active form. Our findings suggest that C. glutamicum has potential as a host for industrial-scale protein production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.