ArticleApplied and environmental microbiology2003
Induction of sucrose utilization genes from Bifidobacterium lactis by sucrose and raffinose.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 87 citations in OpenAlex.
- Advancements in the Heterologous Expression of Sucrose Phosphorylase and Its Molecular Modification for the Synthesis of Glycosylated Products.Molecules (Basel, Switzerland) · 2024Review
- Engineered Sucrose Metabolism Improves the Smut Disease Suppression Potency of Pseudomonas sp. ST4.Applied and environmental microbiology · 2023Article
- The Pleiotropic Effects of Carbohydrate-Mediated Growth Rate Modifications inMicroorganisms · 2023Article
- Review
- Unraveling the mechanism of raffinose utilization in3 Biotech · 2022Article
- Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae.Biotechnology for biofuels · 2021Article
- Manipulation of Gut Microbiota Using Acacia Gum Polysaccharide.ACS omega · 2021Article
- Carbohydrate-controlled serine protease inhibitor (serpin) production in Bifidobacterium longum subsp. longum.Scientific reports · 2021Article
- Recent Development of ProbioticFrontiers in bioengineering and biotechnology · 2021Review
- Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators.Scientific reports · 2019Article
- Unraveling the subtleties of β-(1→3)-glucan phosphorylase specificity in the GH94, GH149, and GH161 glycoside hydrolase families.The Journal of biological chemistry · 2019Article
- Identification ofThe Journal of biological chemistry · 2018Article
- Bifidobacteria and Their Role as Members of the Human Gut Microbiota.Frontiers in microbiology · 2016Review
- Transcriptional Regulation of Carbohydrate Utilization Pathways in the Bifidobacterium Genus.Frontiers in microbiology · 2016Article
- Experimental determination and characterization of the gap promoter of Bifidobacterium bifidum S17.Bioengineered · 2014Article
- Metabolism of four α-glycosidic linkage-containing oligosaccharides by Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2013Article
- Analysis of predicted carbohydrate transport systems encoded by Bifidobacterium bifidum PRL2010.Applied and environmental microbiology · 2012Article
- Metabolism of oligosaccharides and starch in lactobacilli: a review.Frontiers in microbiology · 2012Article
- Carbohydrate metabolism in Bifidobacteria.Genes & nutrition · 2011Article
- Genomic encyclopedia of sugar utilization pathways in the Shewanella genus.BMC genomics · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The probiotic organism Bifidobacterium lactis was isolated from a yoghurt starter culture with the aim of analyzing its use of carbohydrates for the development of prebiotics. A sucrose utilization gene cluster of B. lactis was identified by complementation of a gene library in Escherichia coli. Three genes, encoding a sucrose phosphorylase (ScrP), a GalR-LacI-type transcriptional regulator (ScrR), and a sucrose transporter (ScrT), were identified by sequence analysis. The scrP gene was expressed constitutively from its own promoter in E. coli grown in complete medium, and the strain hydrolyzed sucrose in a reaction that was dependent on the presence of phosphates. Primer extension experiments with scrP performed by using RNA isolated from B. lactis identified the transcriptional start site 102 bp upstream of the ATG start codon, immediately adjacent to a palindromic sequence resembling a regulator binding site. In B. lactis, total sucrase activity was induced by the presence of sucrose, raffinose, or oligofructose in the culture medium and was repressed by glucose. RNA analysis of the scrP, scrR, and scrT genes in B. lactis indicated that expression of these genes was influenced by transcriptional regulation and that all three genes were similarly induced by sucrose and raffinose and repressed by glucose. Analysis of the sucrase activities of deletion constructs in heterologous E. coli indicated that ScrR functions as a positive regulator.
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