ArticleJournal of bacteriology2003
A cka-gfp transcriptional fusion reveals that the colicin K activity gene is induced in only 3 percent of the population.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Specialized Listeria monocytogenes produce tailocins to provide a population-level competitive growth advantage.Nature microbiology · 2024Article
- Heterogeneity in the spontaneous induction of the promoter of the ColE9 operon in Escherichia coli.Archives of microbiology · 2022Article
- Colicin E2 expression in Escherichia coli biofilms: Induction and regulation revisited.Current research in microbial sciences · 2022Article
- Relatedness and the evolution of mechanisms to divide labor in microorganisms.Ecology and evolution · 2021Article
- Article
- The Evolution of Mass Cell Suicide in Bacterial Warfare.Current biology : CB · 2020Article
- A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota.Nature communications · 2019Article
- Plastic responses to competition: Does bacteriocin production increase in the presence of nonself competitors?Ecology and evolution · 2018Article
- Bet-hedging in bacteriocin producing Escherichia coli populations: the single cell perspective.Scientific reports · 2017Article
- Article
- Bacteriocin expression in sessile and planktonic populations of Escherichia coli.The Journal of antibiotics · 2015Article
- Amount of colicin release in Escherichia coli is regulated by lysis gene expression of the colicin E2 operon.PloS one · 2015Article
- Reporters for Single-Cell Analysis of Colicin Ib Expression in Salmonella enterica Serovar Typhimurium.PloS one · 2015Article
- The Escherichia coli uropathogenic-specific-protein-associated immunity protein 3 (Imu3) has nucleic acid -binding activity.BMC microbiology · 2014Article
- Article
- A novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and DNA viral systems.Nucleic acids research · 2011Article
- Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogeneous expression.BMC microbiology · 2010Article
- DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes.Nucleic acids research · 2009Article
- Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD).Microbial cell factories · 2009Article
- The role of SOS boxes in enteric bacteriocin regulation.Microbiology (Reading, England) · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In prokaryotes, only a few examples of differential gene expression in cell populations have been described. Colicin production in natural populations of Escherichia coli, while providing a competitive advantage in the natural habitat, also leads to lysis of the toxin-producing cell. Colicin K synthesis has been found to be induced due to an increase in ppGpp (I. Kuhar, J. P. van Putten, D. Zgur-Bertok, W. Gaastra, and B. J. Jordi, Mol. Microbiol. 41:207-216). Using two transcriptional fusions, cka-gfp and cki-gfp, we show that at the single-cell level, the colicin K activity gene cka is expressed in only 3% of the bacterial population upon induction by nutrient starvation. In contrast, the immunity gene cki is expressed in the large majority of the cells. Expression of the cka-gfp fusion in a lexA-defective strain and in a relA spoT mutant strain indicates that differential expression of cka is established primarily at the level of transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.