ArticleJournal of bacteriology2003
Functional and mutational analysis of conjugative transfer region 2 (Tra2) from the IncHI1 plasmid R27.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Identification of a Plasmid-Mediated Tellurite-Resistance Gene Operon Conferring High-Level Tellurite Resistance in Escherichia Albertii.Microbiology and immunology · 2026Article
- Mechanisms of Transmission and Adaptation ofVeterinary sciences · 2025Article
- Article
- A comprehensive list of genes required for the efficient conjugation of plasmid Rts1 was determined by systematic deletion analysis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- The association between the genetic structures of commonly incompatible plasmids in Gram-negative bacteria, their distribution and the resistance genes.Frontiers in cellular and infection microbiology · 2024Review
- Characterization of anPathogens (Basel, Switzerland) · 2023Article
- Roles of Proteins Containing Immunoglobulin-Like Domains in the Conjugation of Bacterial Plasmids.mSphere · 2022Article
- Protein Dynamics in F-like Bacterial Conjugation.Biomedicines · 2020Review
- Article
- Temperature Dependent Control of the R27 Conjugative Plasmid Genes.Frontiers in molecular biosciences · 2020Article
- Transferable Plasmids ofFrontiers in microbiology · 2020Review
- Expression of a novel class of bacterial Ig-like proteins is required for IncHI plasmid conjugation.PLoS genetics · 2019Article
- Genomic determinants of organohalide-respiration in Geobacter lovleyi, an unusual member of the Geobacteraceae.BMC genomics · 2012Article
- Processing and maturation of the pilin of the type IV secretion system encoded within the gonococcal genetic island.The Journal of biological chemistry · 2011Article
- F-like type IV secretion systems encode proteins with thioredoxin folds that are putative DsbC homologues.Journal of bacteriology · 2005Article
- Temperature-dependent conjugative transfer of R27: role of chromosome- and plasmid-encoded Hha and H-NS proteins.Journal of bacteriology · 2005Article
- A subassembly of R27-encoded transfer proteins is dependent on TrhC nucleoside triphosphate-binding motifs for function but not formation.Journal of bacteriology · 2004Article
- Characterization of the double-partitioning modules of R27: correlating plasmid stability with plasmid localization.Journal of bacteriology · 2003Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transfer 2 region (Tra2) of the conjugative plasmid drR27 (derepressed R27) was analyzed by PSI-BLAST, insertional mutagenesis, genetic complementation, and an H-pilus assay. Tra2 contains 11 mating-pair formation (Mpf) genes that are essential for conjugative transfer, 9 of which are essential for H-pilus production (trhA, -L, -E, -K, -B, -V, -C, -P, and -W). TrhK has similarity to secretin proteins, suggesting a mechanism by which DNA could traverse the outer membrane of donors. The remaining two Mpf genes, trhU and trhN, play an auxiliary role in H-pilus synthesis and are proposed to be involved in DNA transfer and mating-pair stabilization, respectively. Conjugative transfer abilities were restored for each mutant when complemented with the corresponding transfer gene. In addition to the essential Mpf genes, three genes, trhO, trhZ, and htdA, modulate R27 transfer frequency. Disruption of trhO and trhZ severely reduced the transfer frequencies of drR27, whereas disruption of htdA greatly increased the transfer frequency of wild-type R27 to drR27 levels. A comparison of the essential transfer genes encoded by the Tra2 and Tra1 (T. D. Lawley, M. W. Gilmour, J. E. Gunton, L. J. Standeven, and D. E. Taylor, J. Bacteriol. 184:2173-2183, 2002) of R27 to other transfer systems illustrates that the R27 conjugative transfer system is a chimera composed of IncF-like and IncP-like transfer systems. Furthermore, the Mpf/type IV secretion systems encoded by IncH and IncF transfer systems are distinct from that of the IncP transfer system. The phenotypic and ecological significance of these observations is discussed.
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