ArticleJournal of bacteriology2003
Biochemical characterization of a mutationally altered protein translocase: proton motive force stimulation of the initiation phase of translocation.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- The Dynamic SecYEG Translocon.Frontiers in molecular biosciences · 2021Review
- SecA-Mediated Protein Translocation through the SecYEG Channel.Microbiology spectrum · 2019Review
- An energy transduction mechanism used in bacterial flagellar type III protein export.Nature communications · 2011Article
- Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Visualization of distinct entities of the SecYEG translocon during translocation and integration of bacterial proteins.Molecular biology of the cell · 2009Article
- Article
- SecY alterations that impair membrane protein folding and generate a membrane stress.The Journal of cell biology · 2007Article
- Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-cross-linking.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Cloning, expression, purification, crystallization and initial crystallographic analysis of the preprotein translocation ATPase SecA from Thermus thermophilus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2006Article
- Peculiar properties of DsbA in its export across the Escherichia coli cytoplasmic membrane.Journal of bacteriology · 2005Article
- Translation arrest of SecM is essential for the basal and regulated expression of SecA.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Mutational analysis of transmembrane regions 3 and 4 of SecY, a central component of protein translocase.Journal of bacteriology · 2004Article
- Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli.Journal of bacteriology · 2003Article
- A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor of SecY defects.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein translocation across the Escherichia coli plasma membrane is facilitated by concerted actions of the SecYEG integral membrane complex and the SecA ATPase. A secY mutation (secY39) affects Arg357, an evolutionarily conserved and functionally important residue, and impairs the translocation function in vivo and in vitro. In this study, we used the "superactive" mutant forms of SecA, which suppress the SecY39 deficiency, to characterize the mutationally altered SecY39EG translocase. It was found that SecY39-mediated preprotein translocation exhibited absolute dependence on the proton motive force. The proton motive force-dependent step proved to lie before signal peptide cleavage. We suggest that the proton motive force assists in the initiation phase of protein translocation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.