ArticleMolecular and cellular biology2003
The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 123 citations in OpenAlex.
- Menin: from molecular insights to clinical impact.Epigenomics · 2025Review
- Menin in Cancer.Genes · 2024Review
- Review
- Dependency of B-Cell Acute Lymphoblastic Leukemia and Multiple Myeloma Cell Lines on MEN1 Extends beyond MEN1-KMT2A Interaction.International journal of molecular sciences · 2023Article
- Investigating the novel-binding site of RPA2 on Menin and predicting the effect of point mutation of Menin through protein-protein interactions.Scientific reports · 2023Article
- Article
- Wild-type menin is rapidly degraded via the ubiquitin-proteasome pathway in a rat insulinoma cell line.Bioscience reports · 2019Article
- Functional Defects From Endocrine Disease-Associated Mutations in HLXB9 and Its Interacting Partner, NONO.Endocrinology · 2018Article
- The future: genetics advances in MEN1 therapeutic approaches and management strategies.Endocrine-related cancer · 2017Review
- Pro-oncogenic Roles of HLXB9 Protein in Insulinoma Cells through Interaction with Nono Protein and Down-regulation of the c-Met Inhibitor Cblb (Casitas B-lineage Lymphoma b).The Journal of biological chemistry · 2015Article
- Challenges and opportunities in targeting the menin-MLL interaction.Future medicinal chemistry · 2014Review
- GSK-3β protein phosphorylates and stabilizes HLXB9 protein in insulinoma cells to form a targetable mechanism of controlling insulinoma cell proliferation.The Journal of biological chemistry · 2014Article
- FBP1 Is an Interacting Partner of Menin.International journal of endocrinology · 2014Article
- Variable clinical expression in patients with a germline MEN1 disease gene mutation: clues to a genotype-phenotype correlation.Clinics (Sao Paulo, Brazil) · 2012Review
- Trithorax group proteins: switching genes on and keeping them active.Nature reviews. Molecular cell biology · 2011Review
- Crystal structure of menin reveals binding site for mixed lineage leukemia (MLL) protein.The Journal of biological chemistry · 2011Article
- The tumor suppressor protein menin inhibits AKT activation by regulating its cellular localization.Cancer research · 2011Article
- Article
- Menin represses tumorigenesis via repressing cell proliferation.American journal of cancer research · 2011Article
- Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Menin is a 70-kDa protein encoded by MEN1, the tumor suppressor gene disrupted in multiple endocrine neoplasia type 1. In a yeast two-hybrid system based on reconstitution of Ras signaling, menin was found to interact with the 32-kDa subunit (RPA2) of replication protein A (RPA), a heterotrimeric protein required for DNA replication, recombination, and repair. The menin-RPA2 interaction was confirmed in a conventional yeast two-hybrid system and by direct interaction between purified proteins. Menin-RPA2 binding was inhibited by a number of menin missense mutations found in individuals with multiple endocrine neoplasia type 1, and the interacting regions were mapped to the N-terminal portion of menin and amino acids 43 to 171 of RPA2. This region of RPA2 contains a weak single-stranded DNA-binding domain, but menin had no detectable effect on RPA-DNA binding in vitro. Menin bound preferentially in vitro to free RPA2 rather than the RPA heterotrimer or a subcomplex consisting of RPA2 bound to the 14-kDa subunit (RPA3). However, the 70-kDa subunit (RPA1) was coprecipitated from HeLa cell extracts along with RPA2 by menin-specific antibodies, suggesting that menin binds to the RPA heterotrimer or a novel RPA1-RPA2-containing complex in vivo. This finding was consistent with the extensive overlap in the nuclear localization patterns of endogenous menin, RPA2, and RPA1 observed by immunofluorescence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.