ArticleFolia microbiologica2002
Identification and characterization of Clostridium paraputrificum, a chitinolytic bacterium of human digestive tract.
Article in Folia microbiologica, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Myco-foods and the gut microbiome: impacts of mycelial extracts, biomass, and mold-fermented foods.Gut microbes · 2026Review
- Genetic diversity and evolutionary adaptation of Clostridium paraputrificum, a rare pathogen in humans.Communications biology · 2025Article
- Diagnosis and treatment challenges in a rareBiomedical reports · 2024Article
- Metagenomics uncovers dietary adaptations for chitin digestion in the gut microbiota of convergent myrmecophagous mammals.mSystems · 2023Article
- Chitinase Chit62J4 Essential for Chitin Processing by Human Microbiome BacteriumMolecules (Basel, Switzerland) · 2021Article
- Host phylogeny and life history stage shape the gut microbiome in dwarf (Kogia sima) and pygmy (Kogia breviceps) sperm whales.Scientific reports · 2020Article
- Genome Sequence of Clostridium paraputrificum 373-A1 Isolated in Chile from a Patient Infected with Clostridium difficile.Genome announcements · 2016Article
- Colony Location and Captivity Influence the Gut Microbial Community Composition of the Australian Sea Lion (Neophoca cinerea).Applied and environmental microbiology · 2016Article
- Excretome of the chitinolytic bacterium Clostridium paraputrificum J4.Folia microbiologica · 2012Article
- Extracellular complex of chitinolytic enzymes of Clostridium paraputrificum strain J4 separated by membrane ultrafiltration.Folia microbiologica · 2010Article
- Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan.Folia microbiologica · 2008Article
- Effect of chitosan on the growth of human colonic bacteria.Folia microbiologica · 2006Article
- Chitosanase activity in Bacillus thuringiensis.Folia microbiologica · 2004Article
- Detection of cellulolytic bacteria from the human colon.Folia microbiologica · 2004Article
- Chitinolytic enzymes from Clostridium aminovalericum: activity screening and purification.Folia microbiologica · 2004Article
- Biodegradable plastics from renewable sources.Folia microbiologica · 2003Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum. The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas (hydrogen and carbon dioxide) and formation of acetate and lactate (21.6 and 18.9 mmol/L, respectively) and only small quantities of propionate and butyrate (1.7 and 2.6 mmol/L, respectively). In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d (251 nkat/L N-acetyl-D-glucosamine). The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90% of chitin added was degraded within 2 d of cultivation. On the zymogram of the extracellular chitinolytic complex were visible at least 6 isoenzymes with molar mass 43.5-65.0 kDa. The temperature optimum of endochitinase and beta-N-acetylglucosaminidase activities was 50 degrees C; the optimum activity of both enzymes was found at pH 4-6.
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