ArticleJournal of virology2003
The 3C-like proteinase of an invertebrate nidovirus links coronavirus and potyvirus homologs.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 73 citations in OpenAlex.
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- The Structure of the Porcine Deltacoronavirus Main Protease Reveals a Conserved Target for the Design of Antivirals.Viruses · 2022Article
- Recent Progress in Torovirus Molecular Biology.Viruses · 2021Review
- Characterization of Self-Processing Activities and Substrate Specificities of Porcine Torovirus 3C-Like Protease.Journal of virology · 2020Article
- Déjà vu: Stimulating open drug discovery for SARS-CoV-2.Drug discovery today · 2020Review
- Structural basis for catalysis and substrate specificity of a 3C-like cysteine protease from a mosquito mesonivirus.Virology · 2019Article
- A planarian nidovirus expands the limits of RNA genome size.PLoS pathogens · 2018Article
- Complete Genome Sequences of Four Major Viruses Infecting Marine Shrimp in Egypt.Microbiology resource announcements · 2018Article
- Identification and Characterization of a Ribose 2'-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales.Journal of virology · 2016Article
- Discovery of an essential nucleotidylating activity associated with a newly delineated conserved domain in the RNA polymerase-containing protein of all nidoviruses.Nucleic acids research · 2015Article
- Characterization of an alphamesonivirus 3C-like protease defines a special group of nidovirus main proteases.Journal of virology · 2014Article
- CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest.Scientific reports · 2014Article
- Homology modeling and virtual screening for antagonists of protease from yellow head virus.Journal of molecular modeling · 2014Article
- Characterization of Bafinivirus main protease autoprocessing activities.Journal of virology · 2011Article
- Gene expression kinetics of the yellow head virus in experimentally infectedAquaculture research · 2010Article
- Functional and genetic studies of the substrate specificity of coronavirus infectious bronchitis virus 3C-like proteinase.Journal of virology · 2010Article
- Mutation of Glu-166 blocks the substrate-induced dimerization of SARS coronavirus main protease.Biophysical journal · 2010Article
- Genetic diversity in the yellow head nidovirus complex.Virology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gill-associated virus (GAV), a positive-stranded RNA virus of prawns, is the prototype of newly recognized taxa (genus Okavirus, family Roniviridae) within the order NIDOVIRALES: In this study, a putative GAV cysteine proteinase (3C-like proteinase [3CL(pro)]), which is predicted to be the key enzyme involved in processing of the GAV replicase polyprotein precursors, pp1a and pp1ab, was characterized. Comparative sequence analysis indicated that, like its coronavirus homologs, 3CL(pro) has a three-domain organization and is flanked by hydrophobic domains. The putative 3CL(pro) domain including flanking regions (pp1a residues 2793 to 3143) was fused to the Escherichia coli maltose-binding protein (MBP) and, when expressed in E. coli, was found to possess N-terminal autoprocessing activity that was not dependent on the presence of the 3CL(pro) C-terminal domain. N-terminal sequence analysis of the processed protein revealed that cleavage occurred at the location (2827)LVTHE downward arrow VRTGN(2836). The trans-processing activity of the purified recombinant 3CL(pro) (pp1a residues 2832 to 3126) was used to identify another cleavage site, (6441)KVNHE downward arrow LYHVA(6450), in the C-terminal pp1ab region. Taken together, the data tentatively identify VxHE downward arrow (L,V) as the substrate consensus sequence for the GAV 3CL(pro). The study revealed that the GAV and potyvirus 3CL(pro)s possess similar substrate specificities which correlate with structural similarities in their respective substrate-binding sites, identified in sequence comparisons. Analysis of the proteolytic activities of MBP-3CL(pro) fusion proteins carrying replacements of putative active-site residues provided evidence that, in contrast to most other 3C/3CL(pro)s but in common with coronavirus 3CL(pro)s, the GAV 3CL(pro) employs a Cys(2968)-His(2879) catalytic dyad. The properties of the GAV 3CL(pro) define a novel RNA virus proteinase variant that bridges the gap between the distantly related chymotrypsin-like cysteine proteinases of coronaviruses and potyviruses.
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