ArticleThe Journal of cell biology2002
SadA, a novel adhesion receptor in Dictyostelium.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 106 citations in OpenAlex.
- Talin force coupling underlies eukaryotic cell-substrate adhesion.Nature communications · 2025Article
- Reduced cell-substrate adhesion formation promotes cell migration inMolecular biology of the cell · 2025Article
- Reduced cell-substrate adhesion formation promotes cell migration inbioRxiv : the preprint server for biology · 2025Article
- Cell-to-cell signaling in cell populations with large cell size variability.Biophysical journal · 2025Article
- The IQGAP-related RasGAP IqgC regulates cell-substratum adhesion in Dictyostelium discoideum.Cellular & molecular biology letters · 2025Article
- Procedures to Measure Dictyostelium Phagocytosis and Macropinocytosis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Differential cell motion: A mathematical model of anterior posterior sorting.Biophysical journal · 2023Article
- An evolutionary and physiological perspective on cell-substrate adhesion machinery for cell migration.Frontiers in cell and developmental biology · 2022Review
- Adhesion ofFrontiers in cell and developmental biology · 2022Review
- Expanding our understanding of marine viral diversity through metagenomic analyses of biofilms.Marine life science & technology · 2021Article
- Tailored nanotopography of photocurable composites for control of cell migration.RSC advances · 2021Article
- Article
- A telomerase with novel non-canonical roles: TERT controls cellular aggregation and tissue size in Dictyostelium.PLoS genetics · 2019Article
- The putative G protein-coupled receptor GrlD mediates extracellular polyphosphate sensing in Dictyostelium discoideum.Molecular biology of the cell · 2019Article
- Harnessing Motile Amoeboid Cells as Trucks for Microtransport and -Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- A novel mode of cytokinesis without cell-substratum adhesion.Scientific reports · 2017Article
- Shear force-based genetic screen reveals negative regulators of cell adhesion and protrusive activity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Eat Prey, Live:Frontiers in immunology · 2017Review
- Cooperative cell motility during tandem locomotion of amoeboid cells.Molecular biology of the cell · 2016Article
- Interplay between motility and cell-substratum adhesion in amoeboid cells.Biomicrofluidics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Little is known about cell-substrate adhesion and how motile and adhesive forces work together in moving cells. The ability to rapidly screen a large number of insertional mutants prompted us to perform a genetic screen in Dictyostelium to isolate adhesion-deficient mutants. The resulting substrate adhesion-deficient (sad) mutants grew in plastic dishes without attaching to the substrate. The cells were often larger than their wild-type parents and displayed a rough surface with many apparent blebs. One of these mutants, sadA-, completely lacked substrate adhesion in growth medium. The sadA- mutant also showed slightly impaired cytokinesis, an aberrant F-actin organization, and a phagocytosis defect. Deletion of the sadA gene by homologous recombination recreated the original mutant phenotype. Expression of sadA-GFP in sadA-null cells restored the wild-type phenotype. In sadA-GFP-rescued mutant cells, sadA-GFP localized to the cell surface, appropriate for an adhesion molecule. SadA contains nine putative transmembrane domains and three conserved EGF-like repeats in a predicted extracellular domain. The EGF repeats are similar to corresponding regions in proteins known to be involved in adhesion, such as tenascins and integrins. Our data combined suggest that sadA is the first substrate adhesion receptor to be identified in Dictyostelium.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.