ArticleInfection and immunity2003
Identification of LpeA, a PsaA-like membrane protein that promotes cell entry by Listeria monocytogenes.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Translation control by altered start codon usage as a means of modulating the general stress response and virulence in Listeria monocytogenes.PLoS genetics · 2026Article
- Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.World journal of microbiology & biotechnology · 2025Review
- The type of food influences the behaviour of Listeria monocytogenes in a food-gastrointestinal-infection model.NPJ science of food · 2025Article
- Genetic Diversity and Potential Virulence ofFoods (Basel, Switzerland) · 2022Article
- Listeria innocua isolated from diseased ruminants harbour minor virulence genes of L. monocytogenes.Veterinary medicine and science · 2022Article
- Genomic dissection of the most prevalent Listeria monocytogenes clone, sequence type ST87, in China.BMC genomics · 2019Article
- Transcriptome Sequencing ofFrontiers in microbiology · 2019Article
- Review
- How Listeria monocytogenes organizes its surface for virulence.Frontiers in cellular and infection microbiology · 2014Review
- Entry of Listeria monocytogenes in mammalian epithelial cells: an updated view.Cold Spring Harbor perspectives in medicine · 2012Review
- In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection.PLoS pathogens · 2009Article
- Listeria monocytogenes surface proteins: from genome predictions to function.Microbiology and molecular biology reviews : MMBR · 2007Review
- An important step in listeria lipoprotein research.Journal of bacteriology · 2007Article
- Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes.Infection and immunity · 2006Article
- Enhanced synthesis of internalin A in aro mutants of Listeria monocytogenes indicates posttranscriptional control of the inlAB mRNA.Journal of bacteriology · 2005Article
- Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogenicity.Folia microbiologica · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intracellular life of Listeria monocytogenes starts by a complex process of entry involving several bacterial ligands and eukaryotic receptors. In this work, we identified in silico from the sequence of the genome of L. monocytogenes a previously unknown gene designated lpeA (for lipoprotein promoting entry) encoding a 35-kDa protein homologous to PsaA, a lipoprotein belonging to the LraI family and implicated in the cell adherence of Streptococcus pneumoniae and related species. By constructing a mutant of L. monocytogenes in which lpeA is deleted (lpeA mutant), we show that the PsaA-like protein LpeA is not involved in bacterial adherence but is required for entry of L. monocytogenes in eukaryotic cells. In contrast to wild-type bacteria, mutant bacteria failed to invade the epithelial Caco-2 and hepatocyte TIB73 cell lines, as confirmed by confocal microscopy. The mutant bacteria rapidly penetrated in mouse bone marrow-derived macrophages. Surprisingly, lpeA mutant bacteria survive better in macrophages than do wild-type bacteria. This was correlated with a weak exacerbation of virulence of the lpeA mutant in the mouse. LpeA is therefore a novel invasin favoring the entry of L. monocytogenes into nonprofessional phagocytes but not its invasion of macrophages. This is the first report of a lipoprotein promoting cell invasion of an intracellular pathogen.
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