ArticleInfection and immunity2003
Molecular heterogeneity of Ehrlichia chaffeensis isolates determined by sequence analysis of the 28-kilodalton outer membrane protein genes and other regions of the genome.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- A targeted mutational strategy aiding in generating antisense RNA to knockdown theJournal of bacteriology · 2026Article
- Genetic characterization of a novel Ehrlichia chaffeensis genotype from an Amblyomma tenellum tick from South Texas, USA.Ticks and tick-borne diseases · 2022Article
- Comparative Analysis of Genome of Ehrlichia sp. HF, a Model Bacterium to Study Fatal Human Ehrlichiosis.BMC genomics · 2021Article
- Evidence ofInternational journal for parasitology. Parasites and wildlife · 2019Article
- Article
- Extensive genetic diversity of Rickettsiales bacteria in multiple mosquito species.Scientific reports · 2016Article
- Comparative Genomics of the Zoonotic PathogenFrontiers in cellular and infection microbiology · 2016Article
- Immunization with Ehrlichia P28 outer membrane proteins confers protection in a mouse model of ehrlichiosis.Clinical and vaccine immunology : CVI · 2011Article
- Progress and obstacles in vaccine development for the ehrlichioses.Expert review of vaccines · 2010Review
- Anaplasma phagocytophilum and Ehrlichia chaffeensis: subversive manipulators of host cells.Nature reviews. Microbiology · 2010Review
- varDB: common ground for a shifting landscape.Trends in parasitology · 2009Article
- Promoter analysis of macrophage- and tick cell-specific differentially expressed Ehrlichia chaffeensis p28-Omp genes.BMC microbiology · 2009Article
- Molecular characterization of Ehrlichia interactions with tick cells and macrophages.Frontiers in bioscience (Landmark edition) · 2009Review
- Liver transcriptome profiles associated with strain-specific Ehrlichia chaffeensis-induced hepatitis in SCID mice.Infection and immunity · 2009Article
- Total, membrane, and immunogenic proteomes of macrophage- and tick cell-derived Ehrlichia chaffeensis evaluated by liquid chromatography-tandem mass spectrometry and MALDI-TOF methods.Infection and immunity · 2008Article
- Isolation and molecular detection of Ehrlichia from vertebrate animals.Current protocols in microbiology · 2008Article
- Use of Drosophila S2 cells as a model for studying Ehrlichia chaffeensis infections.Applied and environmental microbiology · 2008Article
- Experimental infection of dairy calves with Ehrlichia chaffeensis.Journal of medical microbiology · 2007Article
- Virulence potential of Ehrlichia chaffeensis strains of distinct genome sequences.Infection and immunity · 2007Article
- Diversity of "Candidatus Liberibacter asiaticus," based on the omp gene sequence.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, is responsible for human monocytic ehrlichiosis (HME). In this study, we genetically mapped 10 isolates obtained from HME patients. Sequence analysis of the 28-kDa outer membrane protein (OMP) multigene locus spanning 6 of the 22 tandemly arranged genes identified three distinct genetic groups with shared homology among isolates within each group. Isolates in Groups I and III contained six genes each, while Group II isolates had a gene deletion. There were two regions on the locus where novel gene deletion or insertion mutations occurred, resulting in the net loss of one gene in Group II isolates. Numerous nucleotide differences among genes in isolates of each group also were detected. The shared homology among isolates in each group for the 28-kDa OMP locus suggests the derivation of clonal lineages. Transcription and translation analysis of the locus revealed differences in the expressed genes of different group isolates. Analysis of the 120-kDa OMP gene and variable-length PCR target gene showed size variations resulting from loss or gain of long, direct repeats within the protein coding sequences. To our knowledge this is the first study that looked at several regions of the genome simultaneously, and we provide the first evidence of heterogeneity resulting from gene deletion and insertion mutations in the E. chaffeensis genome. Diversity in different genomic regions could be the result of a selection process or of independently evolved genes.
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